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Cell growth and configuration change checking device based on oxidation/reduction probe, and the checking method

A technology of morphological change and detection device, which is applied in the direction of measuring device, biochemical equipment and method, microbe determination/inspection, etc., can solve the problem of inconvenient use, etc., and achieve the effect of large parameter variation range and high detection sensitivity

Inactive Publication Date: 2007-10-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It first emits a beam of light to the solution. Since the passage of light by ordinary cells is blocked and reflected, the growth and morphological changes of cells in the solution can be measured by measuring the transmitted and reflected light. However, optical methods are used in some colored Not very convenient to use in solution

Method used

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  • Cell growth and configuration change checking device based on oxidation/reduction probe, and the checking method
  • Cell growth and configuration change checking device based on oxidation/reduction probe, and the checking method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The detection of cell growth in the solution of embodiment 1

[0019] Refer to accompanying drawing 1. The present invention includes a reference electrode 1, a generating electrode 2, a collecting electrode 3 and a counter electrode 4, and the distance between the generating electrode and the collecting electrode is less than or equal to 100 microns and greater than or equal to X microns. Accompanying drawing 2 is the implementation schematic diagram that the present invention adopts the interdigitated electrode form, and in the figure, the reference numeral of reference electrode, counter electrode is identical with Fig. 1, and the reference numeral of producing electrode is 5, and the reference numeral of collecting electrode is 6. The present invention is also provided with a double-electrode constant current potentiometer 7 for detecting electrode kinetic parameters on the collecting electrodes.

[0020] Here, the role of the reference electrode is mainly to prov...

Embodiment 2

[0023] The detection of cell growth in the solution of embodiment 2

[0024] The detection device provided in this embodiment is the same as that in Embodiment 1. In the actual test, the device was first immersed in the test solution containing lung fibroblasts (V79), and [Fe(CN) 6 ] 3- / 4- Make the final [Fe(CN) in the solution 6 ] 3- / 4- The concentration is 10mM. Taking the reference electrode as the reference, apply a 0.4V voltage on the generating electrode, and apply a -0.2V DC voltage on the collecting electrode to superimpose an AC voltage with a smaller amplitude. Generally, the AC voltage is less than 10mV, and the frequency used in this embodiment is from 1Hz To a sinusoidal AC voltage with an amplitude of 10mV at 1MHz, the impedance of the electrochemical reaction on the collecting electrode is measured by the standard electrochemical impedance method. At the beginning of the experiment, the electrode impedance measured on the collecting electrode was 400 ohms, ...

Embodiment 3

[0026] The measurement of cell shape change in the solution of embodiment 3

[0027] The structure of the detection device in this embodiment is the same as that in Embodiment 1. In the actual test, the device was first immersed in the test solution containing lung fibroblasts (V79), and [Fe(CN) 6 ] 3- / 4- Make the final [Fe(CN) in the solution 6 ] 3- / 4- The concentration is 10mM. Based on the reference electrode, a voltage of 0.4V was applied to the generating electrode, and a voltage of -0.2V was applied to the collecting electrode, and the current flowing through the collecting electrode was measured to be 300 microamperes. Then, add mercuric chloride to the solution, and make the concentration of mercuric chloride in the final solution be 80 micromolar, and test the current flowing through the collecting electrode, which is 420 microamperes. By comparing the above current values, the cell growth and morphological changes in the tested solution can be deduced, and the e...

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Abstract

The invention provides a device for checking the cell growth and shape change, based on oxidization / reduction probe, comprising a reference electrode, a generating electrode, a collecting electrode, and an opposite electrode. The distance between the generating electrode and the collecting electrode is not larger than 100mum. And the invention also comprises a dual-electrode constant-current potential machine for checking the electrode dynamic parameter of the collecting electrode. The invention also provides a cell growth check method of the device, which comprises that interval measuring the current or electrochemical reaction resistance on the collecting electrode, comparing the data to obtain the result. The invention also provides a cell shape change check method of the device. The invention measures and compares the current or electrochemical reaction resistances on the collecting electrode, after and before adding the agent which can affect the cell growth and shape change, to obtain the agent effect on the cell growth and shape change. The invention has higher sensitivity on the check device and check method.

Description

technical field [0001] The invention relates to an oxidation / reduction probe-based cell growth and morphological change detection device and method. The main application fields of the invention include biochemistry, environmental protection, food safety and the like. Background technique [0002] Cell growth and morphological changes are parameters of great significance in many fields. For example, in the fermentation process, the number of cells in the solution is an important indicator of the fermentation process. In the development of modern new drugs, drug screening is often achieved through the influence of various compounds on the growth, morphology and performance of cells, such as the adhesion of cells and container walls. In environmental detection, the influence of aqueous solutions such as river water and lake water on cell growth is also a common method for environmental monitoring. [0003] At present, the main detection method for c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416C12Q1/02
Inventor 吴坚
Owner ZHEJIANG UNIV