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Reagent kit for forecasting pregnancy badness come-off generating risks

A kit and outcome technology, applied in the field of medicine and biology, can solve the problems of poor pregnancy outcomes relying on clinical experience, low sensitivity and specific indicators, etc.

Active Publication Date: 2007-10-31
深圳泰乐德医学检验实验室
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] In order to overcome the shortcomings of relying on clinical experience and low sensitivity and specificity in predicting adverse pregnancy outcomes, it is necessary to use the genetic marker feature information of biological samples to establish a method for predicting the risk of adverse pregnancy outcomes to help improve prediction efficiency

Method used

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  • Reagent kit for forecasting pregnancy badness come-off generating risks
  • Reagent kit for forecasting pregnancy badness come-off generating risks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1: kit (1) and implementation method thereof (based on PCR-RFLP detection method)

[0078] A kit for predicting the risk of adverse pregnancy outcomes, consisting of the following components:

[0079] (1) Red blood cell lysate: containing NH 4 Cl, KHCO 3 , EDTA;

[0080] (2) White blood cell lysate: containing proteinase K, RNase A, NaCl, Tris, EDTA, SDS;

[0081] (3) Protein precipitation solution: 7.5M ammonium acetate (pH 7.4)

[0082] (4) Nucleic acid storage solution: 1M tris-hydrochloric acid (Tris-HCl, pH8.0);

[0083] (5) PCR reaction mixture: 1.0ml [100mM Tris-HCl, 100mM KCl, pH8.3 (20°C)]; 5.0μMgCl 2 ; Each 0.4mM dATP, dCTP, dGTP, dTTP, prepared in sterile double distilled water, pH 7.0; PCRP gene polymorphic site genotype detection primers;

[0084] (6) Taq DNA polymerase (5U / μl): storage buffer: 20mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% NP40, 0.5% (v / v) Tween 20, 50% glycerin;

[0085] (7) Positive plasmid: a whole blood...

Embodiment 2

[0115] Embodiment 2: kit (2) and implementation method thereof (based on Taqman method)

[0116] A kit for predicting the risk of adverse pregnancy outcomes, consisting of the following components:

[0117] (1) Red blood cell lysate: containing NH 4 Cl, KHCO 3 , EDTA;

[0118] (2) White blood cell lysate: containing proteinase K, RNase A, NaCl, Tris, EDTA, SDS;

[0119] (3) Protein precipitation solution: 7.5M ammonium acetate (pH7.4)

[0120] (4) Nucleic acid storage solution: 1M tris-hydrochloric acid (Tris-HCl, pH8.0);

[0121] (5) PCR reaction mixture: Taq DNA polymerase and its reaction buffer; each 0.4mM dATP, dCTP, dGTP, dTTP;

[0122] (6) Polymorphic site genotype detection primers and Taqman probes of PRCP gene;

[0123] (7) Positive plasmid: a whole blood sample of an individual containing a polymorphic site heterozygous genotype of the PRCP gene, or a plasmid containing a polymorphic site heterozygous genotype of the PRCP gene;

[0124] (8) Negative control: do...

Embodiment 3

[0148] Example 3. Prediction of Risk of Adverse Pregnancy Outcomes

[0149] Utilize the kit of the present invention to measure the genotype of the E112D (rs2298668) polymorphism site of the PRCP gene. When the PRCP genotype is 112ED heterozygous or 112DD homozygous mutant, the incidence of adverse pregnancy outcomes is predicted to be higher; the genotype is When 112EE is homozygous, the incidence of adverse pregnancy outcomes is predicted to be lower.

[0150] When pregnant women with chronic hypertension, the above prediction effect is more obvious. That is, when the PRCP genotype of pregnant women with chronic hypertension is 112ED heterozygous or 112DD homozygous mutant, the incidence of adverse pregnancy outcomes is predicted to be higher.

[0151] The above method has been clinically verified, and the patients with adverse pregnancy outcomes are divided into three groups: homozygous wild type group, heterozygous type group, and homozygous mutant type group. Using the ...

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Abstract

This invention provides one agent case by use of blood vessel and endothelium function to adjust its important praline carboxypeptidase gene mononucleotide polymorphism bit E112D gene and conception imperfect rate relationship on the channel to predict the incidence rate, wherein when the gene is of 112EE pure wild type, the incidence rate is low and when gene is of 112ED mixture or 112DD pure burst type the incidence rate is high. This agent case comprises the E112D polymorphism gene parting oligonucleotide and relative agents.

Description

technical field [0001] The present invention relates to a kit for predicting the risk of adverse pregnancy outcomes by using important enzyme gene polymorphisms on the vascular and endothelial function regulation pathways, including threatened preterm birth and premature birth, intrauterine growth retardation and low birth weight, pregnancy-induced hypertension syndrome As well as adverse pregnancy outcomes such as preeclampsia and eclampsia, it belongs to the field of medical biotechnology. Background technique [0002] Reproductive and reproductive health is a worldwide issue involving maternal and child health, family happiness and sustainable population and social development. Threatened and premature birth (Preterm), intrauterine growth retardation (Uterus Growth Retardation, UGR) and low birth weight (Low Birth Weight, LBW), pregnancy induced hypertension syndrome (pregnancy induced hypertension syndrome, PIHs) and other The three major pregnancy adverse outcomes, inc...

Claims

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Application Information

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IPC IPC(8): G01N33/48A61B10/00C12Q1/68
Inventor 邢厚恂张岩王滨燕李志平吴涤王晓斌臧桐华徐希平
Owner 深圳泰乐德医学检验实验室
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