Swine vesicular disease virus like particle using genetic engineering and the method for manufacturing thereof
A technology for swine vesicular disease virus and recombinant baculovirus, which can be applied to biochemical equipment and methods, cells and viruses modified by introducing foreign genetic material, etc., and can solve the problems of inability to form the conformational structure of virus antigenic sites and conformation dependence.
Inactive Publication Date: 2010-12-01
大韩民国
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Problems solved by technology
However, since porcine vesicular disease virus has a densely-packed icosahedral arrangement of 60 protomers, each protomer consists of 4 proteins (VP1, VP2, VP3 and VP) , and most of the antigenic sites are conformation dependent, however, in the case of expressing only the P1 recombinant protein in E. coli, the conformational structure of the viral antigenic site cannot be formed
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The present invention relates to a swine vesicular disease virus-like particle prepared by a gene recombination technique, as well as a preparation method thereof. More particularly, the invention relates to a method for preparing a swine vesicular disease virus-like particle, wherein the structural protein precursor (Pl) gene and 3CD protease gene of swine vesicular disease virus are inserted simultaneously into a baculovirus expression vector and are expressed in insect cells, so that the expressed 3CD protease enzyme digests the structural protein precursor into individual proteins which then form the capsid of swine vesicular disease virus by an self-assembly process.
Description
Porcine vesicular disease virus-like particle constructed by genetic engineering and preparation method thereof technical field The invention relates to porcine vesicular disease virus-like particles prepared by gene recombination technology and a preparation method thereof. More specifically, the present invention relates to a method for preparing porcine vesicular disease virus-like particles, wherein simultaneously the structural protein precursor (P1) gene and the 3CD protease gene in the porcine vesicular disease virus total gene are inserted into the baculovirus expression vector , and expressed in insect cells, so that the expressed 3CD protease hydrolyzes the structural protein precursor into individual proteins, and then the individual proteins undergo a self-assembly process to form a porcine vesicular disease virus capsid. Background technique Vesicular disease of swine is a highly contagious viral disease of porcine characterized by lesions on the coronaryband,...
Claims
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IPC IPC(8): C12N15/79C12N5/16C12N15/86C12N15/09
CPCA61K2039/5258C12N7/00C12N2770/32323C12N15/63C12N15/79
Inventor 崔康锡高荣骏罗珍珠赵南仁
Owner 大韩民国