Intensified tubercle-resisting action of human interleukin 24

A technology of interleukin, pvax1-il-24, applied in the direction of interleukin, cytokines/lymphokines/interferons, animal/human proteins, etc., can solve the problem that DNA vaccines are difficult to play a protective role, and increase survival Time, improve the effect of pathological level

Inactive Publication Date: 2007-11-14
WUHAN UNIV
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AI Technical Summary

Problems solved by technology

However, it is difficult for DNA vaccines to exert a protective effect when injected alone, so how to improve their immune protection is imminent [4]

Method used

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  • Intensified tubercle-resisting action of human interleukin 24
  • Intensified tubercle-resisting action of human interleukin 24

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Experimental program
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Effect test

Embodiment Construction

[0034]The human interleukin 24 provided by the present invention has the nucleotide sequence shown in SEQ ID NO.1, which contains 601 bases of the human interleukin 24 protein molecule (please refer to the sequence list at the end of the article).

[0035] The present invention provides recombinant plasmid pVAX1-IL-24 of human interleukin 24, the construction of which includes amplifying and purifying human IL-24 cDNA from pcDNA3.1-IL-24 by PCR to obtain a purified PCR product, which is constructed into On the eukaryotic expression vector pVAX-1, specifically include the following steps:

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Abstract

The present invention discloses the human interleukin-24 is shown in SEQ ID NO.1 nucleotide sequence, the sequence containing human interleukin-24 protein molecules of 601 base pairs. Human interleukin-24 recombinant plasmid pVAX1-IL-24, the building will include the use PCR IL-24cDNA from pcDNA3.1-IL-24 amplification and purification, in the purified PCR products, to really build nuclear expression vector pVAX-1, also includes recombinant plasmid pVAX1-IL-24 Construction and preparation steps. Construction of the present invention of pVAX1-IL-24 of the eukaryotic expression vector for in vivo expression in the IL-24 protein, a Th1-type cell-mediated immunity, its joint tuberculosis DNA vaccine Ag85B with immunization, than separate DNA vaccine Ag85B immune used with stronger anti-tuberculosis infection role, and enhance Ag85B resistance tuberculosis infection immune protective effect of IL-24 with that as an immune adjuvant, with the increase of anti-tuberculosis immune protection role.

Description

technical field [0001] The invention belongs to the fields of molecular microbiology and molecular and infection immunity, and in particular relates to a kind of human interleukin-24 (Interleukin-24, IL-24) enhancing anti-tuberculosis effect. Background technique [0002] Tuberculosis (Tuberculosis, TB) is an infectious disease that seriously endangers human health, caused by Mycobacterium tuberculosis (MTB) infection. At present, the global tuberculosis epidemic is still very serious. According to the World Health Organization's estimate, there were 8.8 million new tuberculosis cases in 2005, and 7.4 million cases were in Asia and sub-Saharan Africa. A total of 1.6 million people died from tuberculosis, including 195,000 people living with HIV. Among the 22 countries with a high burden of tuberculosis in the world, my country ranks second, second only to India [1] . Bacillus Calmette-Guerin (BCG) vaccination is currently the main measure to prevent tuberculosis, but larg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/24C07K14/54A61K38/20A61P31/06
Inventor 章晓联孙平
Owner WUHAN UNIV
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