Method for preparing anti-oxidant bio-microcapsule

A technology of antioxidant and antioxidant solution, which is applied in the directions of microcapsule preparation, microsphere preparation, antitoxic agent, etc., can solve the problems of resveratrol's easy oxidation photosensitivity, application limitation, etc., and achieves improved acceptability and stability. The effect of stability, wide scope of application, significant social and economic benefits

Inactive Publication Date: 2008-02-20
万向元
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, resveratrol, as a phytoalexin, has a wide range of physiological and pharmacological activities, including anti-oxidation, cardiovascular protection, anti-inflammation, anti-platelet aggregation, anti-cancer and estrogenic functions, but resveratrol is prone to oxidation and Extreme photosensitivity also greatly limits its application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Shake flask cultures of yeast cells were performed as previously described, followed by harvesting and washing of the yeast.

[0059] Add 0.5%-5% Tween-80 by weight, carry out shaking treatment at 40-60° C. for 24-48 hours to fully autolyze, then centrifuge, wash and freeze-dry.

[0060] 1g of yeast cells and 1-20mL of 1% chlorogenic acid aqueous solution are subjected to high-frequency contact at 30-60°C, taken out after 24 hours, centrifuged, washed with water to remove unembedded chlorogenic acid on the surface, and then freeze-dried.

[0061] Finally, the content of antioxidants in the microcapsules was determined by high performance liquid chromatography.

[0062] It should be pointed out that the function of the added autolysis accelerator is to promote cell autolysis. If a high concentration of autolysis accelerator is added, the reaction time will be shorter, and if a low concentration of autolysis accelerator is added, the reaction time will be longer. Therefo...

Embodiment 2

[0064] The steps of culturing, treating yeast and embedding were the same as in Example 1, except that the added autolysis promoter was TritonX-100.

Embodiment 3

[0066] The steps of culturing, treating yeast and embedding are the same as in Example 1, except that the added autolysis promoter is cetyltrimethylammonium bromide.

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PUM

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Abstract

The invention provides a preparing method for the antioxidant biological micro capsule and relates to the yeast cell cultivation, handling and the preparing method for the micro capsule, particularly the invention relates to an antioxidant biological micro capsule which is made by the method. After the modification handling to the cultivated yeast cell in the shaking bottle by the temperature-eighty or TritonX-one hundred, hexadecyl trimethyl ammonium bromide, sodium laurylsulfate, ethyl acetate, ethanol, hydrochloric acid, sodium hydroxide, potassium chloride and sodium chloride, then offcenter, wash and dry the yeast cell; then contact the cytoderm of the yeast with the water soluble chlorogenic acid or the fatsoluble polydatin in high frequency, and then off center and wash out the antioxidant on the surface of the cell, and then dry and the yeast cell micro capsule without antioxidant on the surface is obtained which has an even size and is not adhesive with a strong antioxidant capability. The invention realizes the stabilization of the chlorogenic acid and can effectively reduce the light retrogradation speed of the Polydatin; and the invention can enhance the water solubility by two to three times and has the slow release capability.

Description

technical field [0001] The invention provides a method for preparing antioxidant biomicrocapsules, which relates to the method for culturing and treating yeast cells and preparing microcapsules, and further relates to the antioxidant biomicrocapsules prepared by the method. technical background [0002] In recent years, the disease prevention and health care functions of functional foods have received more and more attention. The unique role of plant antioxidants, especially polyphenolic compounds, in the prevention and treatment of cardiovascular diseases and cancer suppression has made it an important research field. However, the antioxidant itself is unstable, easily oxidized and deteriorated, and some have bad tastes such as bitterness, which greatly limits its wide application. Antioxidants are microencapsulated with suitable wall materials, and isolated from the outside world by forming a protective layer composed of wall materials around them, which can prevent oxida...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02A61K9/52A61K31/216A61K31/05A23L3/3454A23L3/349A23L3/3517C12N1/16A61P39/06
Inventor 饶力群石国荣
Owner 万向元
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