Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation for improving oxidation resistance of soft-shelled turtle peptide in stomach and intestine and preparation method thereof

A technology of anti-oxidation and soft-shelled turtle peptide, which is applied in the functions of food ingredients, food ultrasonic treatment, food science, etc., can solve the problems of unexplained activity changes, difficult to dissolve, difficult to solve peptide taste, etc., to improve stability and anti-oxidation Oxidation, uniform solution, rapid dissolution effect

Active Publication Date: 2021-08-17
浙江清溪健康科技有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN108107018A enzymatically hydrolyzes milk protein, simulates the gastrointestinal digestion process, tracks the degree of hydrolysis and the ACE inhibition rate of digested products, and does not explain the activity changes of peptides before and after gastrointestinal digestion
[0004] In addition, although peptides have many advantages, the taste of peptides after enzymatic treatment has always been a difficult problem to solve. Peptides derived from animals have a strong fishy smell
Whether it is dry powder or after brewing, the fishy smell of soft-shelled turtle peptide will be unacceptable to people who are in contact with it for the first time, so some people try to add other raw materials to improve the taste after brewing, such as patent CN103535801A, which has the function of relieving physical fatigue and improving hypoxia resistance. Stressed health drink and patent CN111374283A, a kind of asparagus superfine powder with anti-fatigue function and its preparation method, but it only covers up the fishy smell after brewing, and few people try to improve the fishy smell of dry powder; in addition, soft-shelled turtle peptide needs to be stirred In order to dissolve quickly, if it is combined with other general solubility raw materials such as plant concentrated powder, etc., the stirring process is extremely dependent on stirring, and it is easy to form small balls during the stirring process, which is difficult to dissolve quickly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation for improving oxidation resistance of soft-shelled turtle peptide in stomach and intestine and preparation method thereof
  • Preparation for improving oxidation resistance of soft-shelled turtle peptide in stomach and intestine and preparation method thereof
  • Preparation for improving oxidation resistance of soft-shelled turtle peptide in stomach and intestine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1) Weigh 20 parts of sealwort powder and 60 parts of soft-shelled turtle peptide powder;

[0066] 2) Sieve the mixed powder in step 1) with a 200-mesh sieve;

[0067] 3) Dissolve 0.8g in 100mL ultrapure water to form a solution with a concentration of 8.0 mg / ml;

[0068] 4) Take the solution prepared in step 3), and use the corresponding kit to measure the DPPH inhibition rate and superoxide anion radical scavenging rate, and explore the effect of pretreatment on oxidation resistance;

[0069] 5) Refer the solution prepared in step 3) to the INFOGEST 2.0 simulated in vitro gastrointestinal digestion system and method, measure the antioxidant activity of the solution after gastrointestinal digestion, and explore the changes in the antioxidant activity of the formula after pretreatment.

Embodiment 2

[0071] 1) Weigh 20 parts of ginseng powder and 60 parts of soft-shelled turtle peptide powder;

[0072] 2) Sieve the mixed powder in step 1) with a 200-mesh sieve;

[0073] 3) Dissolve 0.8 g in 100mL ultrapure water to form a solution with a concentration of 8.0 mg / ml;

[0074] 4) Take the solution prepared in step 3), and use the corresponding kit to measure the DPPH inhibition rate and superoxide anion radical scavenging rate, and explore the effect of pretreatment on oxidation resistance;

[0075] 5) Refer the solution prepared in step 3) to the INFOGEST 2.0 simulated in vitro gastrointestinal digestion system and method, measure the antioxidant activity of the solution after gastrointestinal digestion, and explore the changes in the antioxidant activity of the formula after pretreatment.

Embodiment 3

[0077] 1. Pre-processing

[0078] 1) Mix soft-shelled turtle peptide powder and sealwort powder in proportion: mix 20 parts of sealwort powder and 60 parts of soft-shelled turtle peptide powder in the container;

[0079] 2) Dissolve the mixed powder with pure water: add 100 parts of pure water to the dry powder in step 1);

[0080] 3) Use ultrasonic-assisted dispersing and shearing to dissolve the powder more thoroughly: insert the shearing head into the container in step 2), and place the container in an ultrasonic cleaner. Turn on the shearing machine, control the speed at 10000-20000rpm, turn on the ultrasonic cleaner, set the power to 600W-900W, ultrasonic hertz to 40KHz, and ultrasonic-assisted shearing time to 20-30min;

[0081] 4) Use freeze-drying to obtain powder: put the sheared and dissolved solution on the cold hydrazine of the freeze dryer, the temperature is -20°C to -40°C, and the time is 30-60min, and then put the frozen material into the vacuum freeze dryer ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation for improving oxidation resistance of soft-shelled turtle peptide in the stomach and intestine and a preparation method thereof. The preparation comprises 40-60 parts of soft-shelled turtle peptide powder and 20-80 parts of compound substances, the preparation method combines an ultrasonic-assisted shearing technology, a freeze-drying technology and a superfine grinding technology, the ultrasonic technology is applied to physical shearing by means of the ultrasonic-assisted shearing technology, the solution is more uniform and thorough, and a large amount of shearing time can be saved. Then freeze-drying is performed to obtain fine powder with less activity loss, and finally the diameter of the powder is further reduced by utilizing a superfine grinding technology, so that the powder is more rapidly dissolved and better in flowing property. The soft-shelled turtle peptide and the compound substance are subjected to pretreatment, so that the soft-shelled turtle peptide and the compound substance interact to form a stable particle form, the gastrointestinal digestion effect can be resisted, the antioxidant activity of the peptide is maintained, and the peptide has no significant difference before and after digestion. The added compound substance can improve the taste problem after the soft-shelled turtle peptide is brewed, and the plant taste can cover the fishy smell of the soft-shelled turtle peptide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation for improving the antioxidant activity of soft-shelled turtle peptide in the gastrointestinal tract and a preparation method thereof. Background technique [0002] Peptide, a compound formed by connecting two or more amino acids through peptide bonds, is a type of substance between proteins and amino acids. Peptides from different sources have differences in function, and different molecular weights and structures will have an impact. Peptides with different molecular weights can be obtained after enzymatic hydrolysis of proteins. Peptides are unstable during digestion in the gastrointestinal tract and will be digested by pepsin and trypsin, resulting in changes in molecular weight and structure, resulting in reduced or even lost activity, which is not conducive to Antioxidant peptides exert their antioxidant properties. The article "Effect of Simulated Gas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/39A23L33/18A23L33/10A23L5/30
CPCA23L2/39A23L33/18A23L33/10A23L5/32A23V2002/00A23V2200/30A23V2250/55A23V2300/10A23V2300/48
Inventor 朱炫陈杰王鑫洋石丽华向沙沙沈宇标王勇宣成桥
Owner 浙江清溪健康科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com