Method of dispensing in reaction vessel and reaction vessel processing apparatus

Abstract

It is intended to easily dispense a minute amount of nonvolatile liquid. In a preferred mode, in the dispensing of mineral oil (40) onto reaction solution (170) previously dispensed in probe disposition site (18), liquid drop (40a) of mineral oil (40) is formed at a distal end of chip (70) of nozzle, and the liquid drop (40a) is brought into contact with an inner wall surface of reaction well or with the surface of reaction solution (170) previously dispensed in a reaction well to thereby attain transfer into the reaction well.

Description

Technical field [0001] The present invention relates to a reaction container suitable for various automatic analysis, such as genetic analysis, research or clinical practice in the medical field, etc., for the detection of human-based, animal or plant genomic DNA polymorphisms. It is a reaction container processing device that is sensitive, especially SNP (Single Base Polymorphism). The detected genetic polymorphism test results can be used to diagnose the prevalence and the relationship between the type of administration and the effect and side effects. Background technique [0002] As a method or device for predicting the susceptibility to disease and the like using genetic polymorphism, the following method or device has been proposed. [0003] In order to determine whether the patient is prone to sepsis and / or whether the sepsis progresses rapidly, a nucleic acid sample is collected from the patient, and the type 2 (pattern) allele in the sample or the linkage disequilibrium ...

AI Technical Summary

Problems solved by technology

At this time, the dispensing amount of the non-volatile liquid is as small as about 1 to 10 μL, and the viscosity of the non-volatile liquid is high, and the liquid cannot be separated from the tip of the nozzle, making it difficult to dispense accurately.

If the reaction is carried out in the state where the non-volatile liquid does not cover the upper surface of the reaction solution and is exposed in the reaction well, there is a problem that the reaction solution evaporates during the reaction and measurement with good precision cannot be performed.

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