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Method for preparing beta-corlan artificial antigen

A betamethasone and artificial antigen technology, applied in animal/human proteins, serum albumin, instruments, etc., can solve the problems of high requirements and high cost of test materials, and achieve the effect of simple synthesis steps

Inactive Publication Date: 2008-04-16
JIANGNAN UNIV
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Problems solved by technology

The commonly used detection methods for betamethasone residues in the world today are: thin layer chromatography (TLC), liquid chromatography (LC), gas chromatography (GC), gas-mass spectrometry (GC-MS), liquid-mass spectrometry (LC-MS), etc., but these instrumental analysis methods not only require expensive instruments and equipment, but also have relatively high requirements for inspection materials, and require complex sample pretreatment before they can be carried out.
Research on the immunoassay method of betamethasone has already been carried out abroad, but there is still a blank in this area in China

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  • Method for preparing beta-corlan artificial antigen
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  • Method for preparing beta-corlan artificial antigen

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Embodiment Construction

[0035] (1) Preparation of artificial hapten:

[0036] Weigh 0.32g of betamethasone and 0.24g of succinic anhydride in a 50mL round-bottomed flask, add 5mL of anhydrous pyridine, heat and stir the mixture at 60°C for 6h, transfer the reactant to a solution containing 10% hydrochloric acid (v / v) In ice water (4°C), a white precipitate precipitated out, centrifuged to remove pyridine and excess succinic anhydride, washed with deionized water several times, centrifuged, dried in a drying oven and stored.

[0037] Reaction end point monitoring: for the production of silica gel thin-layer plates, weigh 12g of silica gel, dissolve it in 40mL of 0.5% mass concentration sodium hydroxymethyl cellulose solution, fully stir it into a paste with a glass rod, oscillate ultrasonically for 1 minute, and spread evenly Place it in a cool place on a glass plate to dry naturally, put it in an oven at 105°C for 1 hour, and put it in a drying oven for later use.

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Abstract

A preparation method of artificial antigen of betamethasone is provided, which belongs the biochemistry field. The present invention adopts betamethasone as the raw material, and utilizes the reaction between uccinic anhydride and betamethasone to produce betamethasone succinate and obtain the artificial semi-antigen, then the active ester method is used to transform the semi-antigen into a intermediate body of active ester, which is combined with bovine serum to prepare the artificial antigen of betamethasone, i.e. betamethasone bovine serum protein. The present invention synthesizes the artificial antigen of betamethasone antigen, and the prepared product can be used for researches on immunization method with glucocorticoid has simplified and effective synthetic procedures, as well as feasibility to be applied to immune analysis, thereby providing a convenient path for the further research by the people, and being capable of satisfying the domestic needs on the study on the object.

Description

technical field [0001] The invention discloses a method for preparing an artificial antigen of betamethasone, which belongs to the field of biochemical industry. Background technique: [0002] Betamethasone (BET) is a synthetic glucocorticoid with the chemical name 16α-methyl-11β, 17α, 21-trihydroxy-9α-fluoropregna-1,4-diene-3,20- diketone. It has the functions of anti-allergy, anti-inflammation and affecting sugar metabolism, and is often used to treat inflammatory reactions, immune diseases, ketosis of cattle and toxemia of sheep pregnancy in livestock. Betamethasone is also commonly used as a growth promoter to increase the feed intake of livestock to achieve weight gain. However, it has been proved by toxicology tests that the drug has mutagenicity and cumulative toxicity, with an ADI value of 0.000015 mg / kgbw / day. If the drug is randomly added to the feed, the accumulated drug will enter the human body through the food chain, which will cause great harm. For this re...

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Application Information

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IPC IPC(8): C07K14/765G01N33/531G01N33/536C07J5/00
Inventor 胥传来袁媛彭池方徐一平刘丽强
Owner JIANGNAN UNIV
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