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Fujian cypress somatic cell embryogenesis and plant regeneration technique

A technology of embryogenesis and somatic embryos, applied to plant cells, etc.

Inactive Publication Date: 2008-04-23
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0023] However, there is no report on the research results of somatic embryogenesis and cloned plant regeneration of Phytophthora fukiensis

Method used

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  • Fujian cypress somatic cell embryogenesis and plant regeneration technique
  • Fujian cypress somatic cell embryogenesis and plant regeneration technique

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Experimental program
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Effect test

Embodiment Construction

[0093] The test materials were zygotic embryos of different developmental degrees of fine individuals of Fukien cypress. The cones are collected from the end of June to the beginning of October, and the corresponding developmental stages of zygotic embryos are from immature embryos to mature embryos. After the cones were collected, they were wrapped in fresh melon leaves, moist gauze and kraft paper envelopes to keep fresh, and then stored in a refrigerator at 4°C for later use.

[0094] Take out the spare cones, soak them with detergent-containing lotion for 10 minutes, and then rinse them with running water for 2 hours. Cut the cone with a scalpel on an ultra-clean workbench, remove the winged seeds with tweezers, and remove the seed wings. Peel off the seed coat and endosperm with a scalpel and tweezers, soak in 75% alcohol for 45 seconds, rinse with sterile water 3 times, disinfect with 0.1% mercury liter + 10 times diluted 84 disinfectant (1:1) for 5 minutes, Rinse 5 ti...

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Abstract

The somatic embryogenesis and cloned plant regeneration process for Fujian cypress includes the steps of inducing embryonic suspensor cell group with immature zygotic embryo, sustaining, proliferation, pre-maturation, maturation to obtain somatic embryo, and germinating culture to obtain regenerated plant. The minimal culture mediums for different stages are DCR minimal medium and improved DCR minimal medium with halved macro elements. The present invention makes it possible to obtain great amount of cloned regenerated plants with identical genetic base, and provides new technological process of providing great amount of Fujian cypress seedlings in short period, high efficiency and low cost.

Description

1. Technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture in forestry, and specifically relates to a somatic cell embryogenesis and plant regeneration technology of Fujian cypress. 2. Background technology [0002] Fujian cypress (Fokienia hodginsii (Dunn) Henry et Thomas), also known as Dushan, Dushu, Jiancypress, Diancypress, etc., belongs to the gymnospermae (Gymnospermae) Cupressacaea) Fujian cypress (Fokienia Henry et Thomus) ). It is the only plant of the genus Fujian Cypress, which is mainly produced in south-central China, south to southwest my country, and is also distributed in Vietnam and Laos. It is one of the first batch of rare tree species under secondary key protection in my country. [0003] Fujian cypress is an evergreen tree with a height of up to 20m and a diameter at breast height of up to 80cm. The crown is broadly conical, the bark is reddish brown, nearly smooth, and falls off in flakes. , th...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 施季森陈加飞
Owner NANJING FORESTRY UNIV
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