Method for rapidly propagating aralia elata through somatic embryo and secondary somatic embryogenesis
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A technique of multiplying Aralia japonica and somatic embryos, applied in the field of agricultural biology, can solve the problems of low variability, cumbersome regeneration process, and large variability
Inactive Publication Date: 2009-10-07
NORTHEAST FORESTRY UNIVERSITY +1
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The plant regeneration process is relatively cumbersome and highly variable
The r...
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example 1
[0024] Example 1 Induction of Somatic Embryogenesis of Aralia longya and Regeneration of its Plants
[0025] 1) Material source and lamination treatment
[0026] The seeds of Aralia longya were collected from the seed garden of Heilongjiang Forestry Diversification Research Institute (Mudanjiang City). Because the zygotic embryos in the mature fruits of Aralia longya have not matured, there is dormancy in seed germination. To break the dormancy, post-ripening treatment of zygotic embryos must be carried out. The treatment method we use is stratification treatment, that is, the seeds are mixed with wet sand after a simple disinfection treatment, and placed in a refrigerator at 4°C.
[0027] 2) Obtaining sterile seedlings
[0028] After 4 months of seed stratification, select the pod-opened seeds with mature zygotic embryos, take out the zygotic embryos after routine disinfection in the ultra-clean workbench, and inoculate them in the 1 / 2 MS medium prepared in advance to germi...
example 3
[0037] Example 3 Induction of Secondary Somatic Embryogenesis and Plant Regeneration
[0038] 1) Induction of secondary somatic embryos
[0039] Select somatic embryos with a length of about 1 cm, inoculate them in SH medium containing different concentrations of sucrose (1-7%), and SH medium containing different concentrations (0.2-3.0 mg / L) of IBA and 2% sucrose Induction of secondary somatic embryos. Each treatment contained 5 Petri dishes inoculated with 12 explants per Petri dish. Dark culture conditions.
[0040] After 4 weeks, the statistical results (see the table below) showed that IBA could promote the formation of secondary somatic embryos more than sucrose, and its optimal concentration was 3.0 mg / L.
[0041] Effects of Sucrose and IBA on Secondary Somatic Embryogenesis
[0042]
[0043] 2) Secondary somatic embryo formation and observation
[0044] During the formation of secondary somatic embryos, somatic embryos at different developmental stages could be...
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Abstract
The present invention provides a method for rapidly propagating aralia elata through somatic embryo and secondary somatic embryogenesis. The invention uses the leaf, petiole and root segment of young seedling of aralia elata as explants. The explants are dark-cultivated for 35 days in the SH cultivation with 3.0mg/L, 2.0mg/L and 0.3mg/L of IBA concentration respectively and 2% of sucrose. The somatic embryo inductivities of three explants can equally obtain 100%. Furthermore each explant generates more somatic embryos (8.6-11.3). The sucrose concentration and IBA can equally affect the generation of secondary somatic embryo. After the explants are dark-cultivated for 4 weeks in the cultivation condition of 3.0mg/L IBA+ 2% of sucrose+ SH culture medium, 100% of somatic embryos are generated with secondary somatic embryos. The obtained mature primary somatic embryos and secondary somatic embryos are transferred to a WPM culture medium with no hormone. The mature primary somatic embryos and secondary somatic embryos can quickly germinate and grow to seedling. The robust seedling with length about 5cm is transplanted into the soil, and the survival rate can obtain 89%. The method which is provided by the invention and has the advantages of simple culturing, short regenerate cycle and capability for continuously obtaining the regenerated plant can be used for the industrial production of aralia elata seedling.
Description
technical field [0001] The invention relates to a method for simple and rapid asexual propagation of Aralia japonica through somatic cell generation and secondary somatic cell generation. It mainly uses an exogenous hormone (IBA) to induce somatic embryos and secondary somatic cells from the leaves, petioles and roots of seedlings, and obtain a large number of regenerated seedlings. The invention belongs to the field of agricultural biotechnology. Background technique [0002] Aralia elate Seem., a small tree belonging to the Araliaceae genus Aralia, is a plant with both food and medicine. Its sprouts contain various amino acids and trace elements necessary for the human body. It is a good health food and one of the wild vegetable varieties that are mainly exported to earn foreign exchange in my country [Zhao Hengtian et al., Agricultural System Science and Comprehensive Research, 2004]. Aralia japonica is also an important medicinal resource. Its extract has anti-inflamm...
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