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Flexible culture medium bag containing nutrient concentrate

A concentrate and culture medium technology, applied in the direction of microbial measurement/inspection, biomass post-treatment, biomass pre-treatment, etc., can solve problems such as excessive complexity

Inactive Publication Date: 2008-05-28
EI DU PONT DE NEMOURS & CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many media types can lead to overly complex inventory of prefilled bags for production and distribution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] By applying 13.6 g of DuPont Qualicon BAX for Listeria  (Suitable for preparing enrichment concentrates suitable for culturing Listeria bacteria) Dissolve in sufficient water to achieve a total volume of 60ml to prepare a liquid nutrient concentrate. Other batches of different volumes were similarly prepared by adjusting the amounts and types of nutrients and the ratio of water.

Embodiment 2

[0097] Five-layer coextruded blown film is produced on a five-layer blown film line to make the outer layer of LDPE with a melt index of 0.3 and a density of 0.918 g / cc, and adjacent anhydride-modified PE (Bynel  4104) adhesive layer, ethylene vinyl alcohol (EVOH; Eval F101A) barrier layer, anhydride-modified PE (Bynel  41 E687) a second adhesive layer, and an inner sealant layer containing a melt: 11 wt% random polypropylene copolymer with a melt flow rate of 7, a melting point of 135°C, and 89 wt% ethylene ion A polymer terpolymer containing 10 wt% methacrylic acid and 10 wt% isobutyl acrylate, wherein 17% of the acid groups were neutralized by zinc. The LDPE was melted at 219°C in a 63.5 mm single screw extruder operating at 62 rpm. The EVOH was melted at 211 °C in a 50.8 mm single screw extruder operating at 27 rpm. Bynel was melted at 215°C in a 50.8 mm single screw extruder running at 34 rpm  4101. Bynel was melted at 196°C in a 50.8 mm single screw extruder runni...

Embodiment 3

[0099] Open the homogeneous bag that contains the sachet in embodiment 2, add 25g food sample and 165ml sterile water (to provide the nutrient medium that total volume is 225ml to guarantee that the ratio of final sample and nutrient medium is the 1 that the nutrient manufacturer specifies :9). The bag was placed in a homogenizer with a reciprocating paddle and mixed for one minute, thereby breaking the frangible seal and dispersing the nutrient concentrate into the added water. The samples in the bags were then incubated at 36°C for 24 hours. After incubation, a portion of the growth medium was removed and analyzed for the presence of Listeria using rapid method procedures such as enzyme immunoassay, gene probe detection or by Bacteriological Analytical Manual (FDA, 8th edition, hereafter referred to as "BAM") as part of a method for the detection of this organism in a 25 g food sample.

[0100] Different pathogenic bacteria such as E. coli 0157:H7, Staphylococcus aureus, S...

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PUM

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Abstract

Disclosed is a culture medium container such as a bag comprising a main compartment and a locus of containment that contains a nutrient concentrate until it is released at the time of use. The locus of containment may comprise separated compartments defined by frangible seals or seals comprising a water-reactive material. Sachets comprising frangible seals or a water-reactive material are also suitable for the locus of containment. Matrices and coatings comprising water-reactive material are also suitable. Capsules that can be pulverized and / or dissolved may also be used.

Description

[0001] The present invention relates to a media bag comprising a main compartment and a locus of containment containing a nutrient concentrate which is released upon use. Background technique [0002] Microorganisms can be present in food and the environment in low concentrations that are difficult to measure and still pose a significant health threat. Microbiologists incubate samples in liquid media to detect and perform tests for pathogenic microorganisms such as those found in Salmonella, Listeria, Staphylococcus, Clostridium, Campylobacter, and Microorganisms of the genus Escherichia. A similar type of test is performed to detect the presence of microorganisms in samples normally expected to be sterile, such as blood, spinal fluid, medical devices, and various industrial materials. [0003] To increase the concentration of microorganisms to measurable levels, the sample for analysis is mixed with a nutrient medium in which the population of organisms is grown. This growt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/06C12Q1/10
CPCC12M23/14C12Q1/04C12M23/34
Inventor J·C·斯泰肯B·W·安达洛罗小J·P·凯恩D·L·维肖利S·王
Owner EI DU PONT DE NEMOURS & CO
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