Washing method and device for biological chip

Abstract

The invention relates to a cleaning method of biochip and device, which comprises the following procedures: firstly, a plurality of fixing grooves fixed with a biochip are arranged in a cleaning chamber; secondly, at least a pipeline to a liquid cleaning bottle and a pipeline to a waste liquid bottle are connected under the cleaning chamber, a liquid pump and a valve are arranged on the pipeline; thirdly, the valve to the waste liquid bottle is closed, the valve to the cleaning liquid is opened, through liquid pump, make the cleaning liquid pump in the cleaning chamber; fourthly, the valve to the cleaning liquid is closed and the valve to the waste liquid bottle is opened. Starts the liquid pump for / backward frequently, and makes the cleaning liquid pump in the cleaning chamber enter into the waste liquid bottle by pump. The invention has simple structure and lower cost, which realizes the automatic cleaning to biochip, avoids the error caused by manual operation, and is easier to realize automatic controlling and integrating.

Description

technical field [0001] The invention relates to a cleaning method and device, in particular to a biological chip cleaning method and device. Background technique [0002] There are mainly two types of existing biochip washing methods, one is to use an instrument for cleaning, and the other is to use a shaking table for washing. [0003] There are two main types of instrument cleaning: one is a special cleaning instrument, such as the AdvaWash AW400 slide washer produced by Advalytix, which can clean general-purpose slides and four chips at the same time. The automatic cleaning of the chip is achieved, but it cannot meet the requirement that some chips must be cleaned under certain temperature conditions. The other is to use the washing device that comes with the hybridization instrument to wash. For example, the Lucidea SlidePro hybridization instrument produced by Amersham Biosciences can hybridize six chips at the same time. After hybridization, the chip can be cleaned an...

AI Technical Summary

Problems solved by technology

Compared with washing on copper racks, the washing of chips in plastic boxes is not conducive to large-scale washing and centralized centrifugal drying

When the chip is washed on the copper rack in the beaker, the difference in the flow rate of the washing liquid between the upper and lower layers of the copper rack is increased, and it is easy to affect the chip. In addition, the difference in the amount of washing liquid in the beaker has a relatively large impact on the cleaning speed, which greatly reduces Improved reproducibility of chip cleaning

For these two shaking table washing methods, if the chip needs to be cleaned by more than two cleaning solutions, the process of changing the cleaning solution still needs to be manually operated, and the errors caused by manual work will further reduce the repeatability of chip cleaning. sex

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