Procambarus clarki ovum in-vitro breeding technique
A technology of prawn crayfish and shrimp eggs is applied in the field of in vitro cultivation of prawn crayfish eggs to achieve the effects of improving survival rate, convenient transportation and high hatching rate
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Embodiment 1
[0016] Embodiment one: put the ovum-bearing shrimp in the bleaching powder solution of 0.5mg / L and soak for 10 minutes, gently pick off the shrimp ovum of the ovum-bearing shrimp abdomen with sterile tweezers, put the shrimp ovum in the same developmental stage on the seedbed, and use Shrimp eggs were submerged in sterilized river water with a pH value of 6.8, the immersion depth was 5 cm, the temperature of the river was 15 degrees, the light intensity was 1000 Lux, and the light was 6 hours. After 10 days of cultivation, the hatching rate of shrimp eggs was 41.2%.
Embodiment 2
[0017] Embodiment two: the ovum-bearing shrimp is placed in the bleaching powder solution of 0.5mg / L and soaks 15 minutes, plucks the ovum of ovum-bearing shrimp abdomen gently with sterile tweezers, the shrimp ovum that is in the same developmental stage is placed on the seedling bed, uses Shrimp eggs were submerged in sterilized river water with a pH value of 8.2. The immersion depth was 50 cm, the temperature of the river water was 20 degrees, and the light intensity was 500 Lux for 8 hours. After 10 days of cultivation, the hatching rate of shrimp eggs was 53%.
Embodiment 3
[0018] Embodiment three: put the ovum-bearing shrimp in the bleaching powder solution of 0.5mg / L and soak for 30 minutes, gently pick off the shrimp ovum of the ovum-bearing shrimp abdomen with sterile tweezers, put the shrimp ovum in the same developmental stage on the seedbed, and use Shrimp eggs were submerged in river water with a sterilized pH value of 7.2. The immersion depth was 100 cm, the temperature of the river was 25 degrees, and the light intensity was 4000 Lux for 12 hours. After 10 days of cultivation, the hatching rate of shrimp eggs was 67.2%.
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