Procambarus clarki ovum in-vitro breeding technique

A technology of prawn crayfish and shrimp eggs is applied in the field of in vitro cultivation of prawn crayfish eggs to achieve the effects of improving survival rate, convenient transportation and high hatching rate

Inactive Publication Date: 2008-07-09
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the principles of developmental biology and the applicant's research results on the embryonic development process of Procambarus clarkii, the present invention proposes a technique for

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment one: put the ovum-bearing shrimp in the bleaching powder solution of 0.5mg / L and soak for 10 minutes, gently pick off the shrimp ovum of the ovum-bearing shrimp abdomen with sterile tweezers, put the shrimp ovum in the same developmental stage on the seedbed, and use Shrimp eggs were submerged in sterilized river water with a pH value of 6.8, the immersion depth was 5 cm, the temperature of the river was 15 degrees, the light intensity was 1000 Lux, and the light was 6 hours. After 10 days of cultivation, the hatching rate of shrimp eggs was 41.2%.

Embodiment 2

[0017] Embodiment two: the ovum-bearing shrimp is placed in the bleaching powder solution of 0.5mg / L and soaks 15 minutes, plucks the ovum of ovum-bearing shrimp abdomen gently with sterile tweezers, the shrimp ovum that is in the same developmental stage is placed on the seedling bed, uses Shrimp eggs were submerged in sterilized river water with a pH value of 8.2. The immersion depth was 50 cm, the temperature of the river water was 20 degrees, and the light intensity was 500 Lux for 8 hours. After 10 days of cultivation, the hatching rate of shrimp eggs was 53%.

Embodiment 3

[0018] Embodiment three: put the ovum-bearing shrimp in the bleaching powder solution of 0.5mg / L and soak for 30 minutes, gently pick off the shrimp ovum of the ovum-bearing shrimp abdomen with sterile tweezers, put the shrimp ovum in the same developmental stage on the seedbed, and use Shrimp eggs were submerged in river water with a sterilized pH value of 7.2. The immersion depth was 100 cm, the temperature of the river was 25 degrees, and the light intensity was 4000 Lux for 12 hours. After 10 days of cultivation, the hatching rate of shrimp eggs was 67.2%.

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Abstract

The invention put forwards an excised cultivation technology of procambarus clakii eggs according to developmental biology principle. The key points of the technology are the separation between parent prawns and eggs of berried prawns, the classification of the eggs, and respectively artificial hatching of the eggs. The steps of the invention are as follows: firstly, the berried prawns are sterilized; secondly, the parent prawns and the eggs are separated; thirdly, the eggs in different development stages are classified; fourthly, nidation of the eggs; fifthly, the hatching of the eggs. The invention has a high egg hatching rate, uniform sized young hatching prawns and a high commodity value. The cultivation technology can effectively prevent the young prawns from eating each other; therefore the survival rate of the young prawns is improved. Because the berried prawns do not need to be reared, cultivation cost is reduced and the transportation of prawn eggs is more convenient.

Description

technical field [0001] The invention relates to aquaculture technology, in particular to the in vitro cultivation technology of Procambarus clarkii eggs. Background technique [0002] Procambarus clarkii (the scientific name is also called "crawfish clarkii", commonly known as "lobster", "crayfish", hereinafter referred to as "lobster".) Lobster, since it was cooked and made into delicious food, the market demand has become more and more Big. Especially in recent years, wild lobsters have become an important factor restricting the development of the lobster industry due to a large number of catches and the gradual depletion of resources. In order to solve the above problems, aquatic science and technology personnel have conducted research on the artificial propagation technology of lobster, which includes the cultivation of shrimp seedlings, the survival and growth of shrimp larvae, and the reproduction of adult shrimp, etc. At present, the breeding method of shrimp seedli...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 丁怀宇潘正军周雪瑞杨占军钱姗姗赵永华
Owner HUAIYIN TEACHERS COLLEGE
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