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Recombined foot and mouth disease virus amalgamation protein vaccine

A foot-and-mouth disease virus and recombinant protein technology, applied in the field of genetic engineering recombinant protein vaccine, recombinant foot-and-mouth disease virus fusion protein vaccine for preventing animal foot-and-mouth disease virus infection and its preparation, can solve problems such as insufficient inactivation and foot-and-mouth disease

Inactive Publication Date: 2008-08-20
BEIJING HYDVAX BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional foot-and-mouth disease vaccine is an inactivated vaccine made by emulsifying the initially purified inactivated foot-and-mouth disease virus with an adjuvant. It has a considerable protective effect on susceptible livestock, but it may cause foot-and-mouth disease due to insufficient inactivation

Method used

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  • Recombined foot and mouth disease virus amalgamation protein vaccine
  • Recombined foot and mouth disease virus amalgamation protein vaccine
  • Recombined foot and mouth disease virus amalgamation protein vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1 Synthetic construction of recombinant foot-and-mouth disease virus fusion protein (A2) encoding gene

[0097] 1. Synthesis of 1A2 coding gene DNA fragment 1

[0098] The DNA fragment 1 of the gene encoding recombinant foot-and-mouth disease virus fusion protein A2 was synthesized by two rounds of PCR. PCR primers (synthesized by Shanghai Sangon Bioengineering Co., Ltd.) with the following sequences were designed and synthesized:

[0099] Primer 1: 5′TACGGTGAAGAATCTTCTCGCCGCGGTGACCTGGCAGCACTGGCACGTCGTGTTAA3′

[0100] Primer 2: 5′ ACGAGCGGTCTGAGTTTCGCCACCAGAGGTCGGCAGACGGTTGTTAACACGACGTGCCA 3′

[0101] Primer 3: 5′GAATTCAGATCTGGCGGCTACAACGGCAAAACCACCTACGGTGAAGAATCTTCT3′

[0102] Primer 4: 5′ AAGCTTGGATCCAACGAAACGGTCCAGAACGAAAGCAACATCAGTGTGCAGGCGACGAGCGGTCTGAGTTTC 3′

[0103] Use primer 1 and primer 2 as templates for the first round of PCR. The first round of PCR reaction conditions: 95°C, 45s; 55°C, 1min; 72°C, 2min; 5 cycles, 72°C extension 10min after ...

Embodiment 2

[0118] Construction of embodiment 2 recombinant foot-and-mouth disease virus fusion protein (A6) coding gene

[0119] 2.1 Synthesis of DNA Fragment 1 of A6 Encoding Gene

[0120] The DNA fragment 1 of the gene encoding recombinant foot-and-mouth disease virus fusion protein A6 was synthesized by two rounds of PCR. PCR primers (synthesized by Shanghai Sangon Bioengineering Co., Ltd.) with the following sequences were designed and synthesized:

[0121] Primer 1: 5′TACGGTGAAGAATCTTCTCGCCGCGGTGACCTGGCAGCACTGGCACGTCGTGTTAA3′

[0122] Primer 2: 5′ ACGAGCGGTCTGAGTTTCGCCACCAGAGGTCGGCAGACGGTTGTTAACACGACGTGCCA 3′

[0123] Primer 3: 5′GAATTCAGATCTGGCGGCTACAACGGCAAAACCACCTACGGTGAAGAATCTTCT3′

[0124] Primer 4: 5′ AAGCTTGGATCCAACGAAACGGTCCAGAACGAAAGCAACATCAGTGTGCAGGCGACGAGCGGTCTGAGTTTC 3′

[0125] The PCR reaction conditions used, the method for recovering and cloning the recombinant foot-and-mouth disease virus fusion protein A6 encoding gene DNA fragment 1 are as in 1.1 of Example 1....

Embodiment 3

[0145] Embodiment 3 Synthetic construction of recombinant foot-and-mouth disease virus fusion protein (A7) encoding gene

[0146] 3.1 Synthesis of DNA Fragment 1 of A7 Encoding Gene

[0147] The DNA fragment 1 of the gene encoding recombinant foot-and-mouth disease virus fusion protein A7 was synthesized by two rounds of PCR. PCR primers (synthesized by Shanghai Sangon Bioengineering Co., Ltd.) with the following sequences were designed and synthesized:

[0148] Primer 1: 5′TACGGTGAAGAATCTTCTCGCCGCGGTGACCTGGCAGCACTGGCACGTCGTGTTAA3′

[0149] Primer 2: 5′ ACGAGCGGTCTGAGTTTCGCCACCAGAGGTCGGCAGACGGTTGTTAACACGACGTGCCA 3′

[0150] Primer 3: 5′GAATTCAGATCTGGCGGCTACAACGGCAAAACCACCTACGGTGAAGAATCTTCT3′

[0151] Primer 4: 5′ AAGCTTGGATCCAACGAAACGGTCCAGAACGAAAGCAACATCAGTGTGCAGGCGACGAGCGGTCTGAGTTTC 3′

[0152] The PCR reaction conditions used, the method for recovering and cloning the recombinant foot-and-mouth disease virus fusion protein encoding gene DNA fragment 1 are as in 1.1 of E...

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Abstract

The invention provides a restructured foot-mouth disease virus fusion protein vaccine designed according to Asian I-type foot-mouth disease virus, experiments proves: the vaccine of the invention produced by gene engineering technique has good immune effect and foot-mouth disease infection prevention. The invention provides the restructured foot-mouth disease virus protein vaccine amino acid sequence, and the research about the nucleotide sequence and foot-mouth disease infection prevention.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a genetic engineering recombinant protein vaccine, in particular to a recombinant foot-and-mouth disease virus fusion protein vaccine for preventing animal foot-and-mouth disease virus infection and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (FMD) is a severe infectious disease caused by foot-and-mouth disease virus. Pigs, cattle, and sheep can all be infected with foot-and-mouth disease virus and develop foot-and-mouth disease. In mild cases, lesions such as blisters, ulcers, and rotten spots appear on the mouth, tongue, lips, hooves, and breasts, and in severe cases, death occurs. The occurrence and prevalence of foot-and-mouth disease will cause huge direct and indirect economic losses (Jiang Pengfei, Zhao Qizu, Xie Qingge, Research Progress of Foot-and-Mouth Disease Chinese Agricultural Sciences 1999, 32(6): 93-100). The Internationa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/135A61K48/00A61P31/14
Inventor 王丽颖任继玲徐海飞张永胜王华于永利
Owner BEIJING HYDVAX BIOTECH
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