Method for producing fuel ethyl alcohol with vapour explosion sweet potato direct fermentation
A technology for fuel ethanol and sweet potato, which is applied in the directions of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve problems such as large consumption, and achieve the effects of increasing the gelatinization rate, reducing the processing process, and reducing the production cost.
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Embodiment 1
[0019] Embodiment 1: get sweet potato 500g, cut into small sections, put into steam explosion tank, process 2.5 minutes under steam pressure 0.5MPa; In the material after treatment, add glucoamylase according to the ratio of adding 100U glucoamylase for every gram of steam explosion material, After saccharification in a 60°C water bath for 40 minutes, cool to 30°C and sterilize by ultraviolet light for 20 minutes. Under sterile conditions, add 0.1% ammonium sulfate, 0.1% potassium dihydrogen phosphate and 0.10% activated yeast, then ferment at 30°C for 60 hours, collect ethanol by distillation, and the alcohol content is 8.89%.
Embodiment 2
[0020] Embodiment 2: get sweet potato 500g, cut into small sections, drop into steam explosion tank, process 2.5 minutes under steam pressure 0.6MPa; In the material after treatment, add glucoamylase according to the ratio of adding 120U glucoamylase per gram of steam explosion material, After saccharification in a water bath at 55°C for 60 minutes, it was cooled to 32°C and sterilized by ultraviolet light for 15 minutes. Under aseptic conditions, 0.15% ammonium sulfate, 0.1% potassium dihydrogen phosphate and 0.25% activated yeast were added, then fermented at 32°C for 54 hours, and ethanol was collected by distillation with an alcohol content of 9.27%.
Embodiment 3
[0021] Embodiment 3: get sweet potato 500g, cut into small sections, put into steam explosion tank, process 2.5 minutes under steam pressure 0.7MPa; In the material after treatment, add glucoamylase according to the ratio of adding 150U glucoamylase for every gram of steam explosion material, After saccharification in a water bath at 58°C for 40 minutes, it was cooled to 35°C and sterilized by ultraviolet light for 18 minutes. Under sterile conditions, add 0.15% ammonium sulfate, 0.15% potassium dihydrogen phosphate and 6.0% activated yeast, then ferment at 35°C for 48 hours, collect ethanol by distillation, and the alcohol content is 12.47%.
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