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Combination cultivation kit of beadroll bacterium, hemophilus and mycoplasma, and uses thereof

A technology for haemophilus and candida, which is applied in the direction of biochemical equipment and methods, microbiological determination/inspection, etc., can solve problems such as adverse effects on accuracy, sensitivity, specificity, and stability defects, and achieve reliable methods and easy operation. Simple and stable effect

Inactive Publication Date: 2008-09-24
叶鹰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This culture method has certain defects in sensitivity, specificity, stability, etc., which will have a negative impact on the accuracy of the final test results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A kind of candida, haemophilus and mycoplasma combination culture kit, specifically as follows:

[0037] 1. Composition, formula and principle

[0038] 1.1 Composition: Medium, CO 2 Happening piece

[0039] 1.2 Formula: Each liter includes the following ingredients, pH is 6.3±0.5 (25±1°C), solid phase plus agar 10~12g

[0040] Basic base (including beef heart powder 10g, peptone 5g, NaCl 5g) 700ml

[0041] Horse (or cow) serum 100ml

[0042] Yeast Extract 4g

[0043] Urea 6g

[0044] L-arginine hydrochloride 3g

[0045] Phenol red 0.5g

[0046] Complex Growth Factors 10ml

[0047] Antibiotics 10ml

[0048] Glucose 10g

[0049] Haemophilus medium plus hemoglobin 20g

[0050] Compound growth factors include 1% DMEM 3ml, 0.5% DNA 3ml and 1% cystine 4ml; the antibiotic for Candida medium is gentamicin, and the antibiotic for Haemophilus medium is vancomycin 5ml and polymyxin 5ml, the antibiotics of mycoplasma culture medium are fluconazole 4ml and penicillin 6ml...

Embodiment 2

[0088] A kind of candida, haemophilus and mycoplasma combination culture kit, including culture medium, CO 2 Slices were produced, and the pH of the medium was 6.3±0.5 (25±1°C).

[0089] The formula (every 1000ml) of described culture medium comprises:

[0090] Basic base (including beef heart powder 20g, peptone 1g, NaCl 2g) 600ml

[0091] Horse (or cow) serum 200ml

[0092] Yeast extract 2g

[0093] Urea 10g

[0094] L-arginine hydrochloride 1g

[0095] Phenol red 0.8g

[0096] Complex Growth Factors 20ml

[0097] Antibiotics 20ml

[0098] Glucose 5g

[0099] Agar 10g

[0100] Haemophilus medium plus 10% serum 20g

[0101] Compound growth factors include 1% DMEM 5ml, 0.5% DNA 7ml and 1% cystine 8ml; the antibiotic for Candida culture medium is chloramphenicol, the antibiotic for Haemophilus culture medium is vancomycin 6ml and polymyxin 14ml , the antibiotics in the mycoplasma medium were fluconazole 5ml and penicillin 15ml.

[0102] The application method of abo...

Embodiment 3

[0111] A kind of candida, haemophilus and mycoplasma combination culture kit, including culture medium, CO 2 Slices were produced, and the pH of the medium was 6.3±0.5 (25±1°C).

[0112] The formula (every 1000ml) of described culture medium comprises:

[0113] Basic base (including beef heart powder 5g, peptone 10g, NaCl 8g) 800ml

[0114] Horse (or cow) serum 50ml

[0115] Yeast Extract 8g

[0116] Urea 2g

[0117] L-Arginine Hydrochloride 5g

[0118] Phenol red 0.1g

[0119] Complex Growth Factor 5ml

[0120] Antibiotic 5ml

[0121] Glucose 20g

[0122]Agar 12g

[0123] Haemophilus medium plus 2% egg yolk 5g

[0124] Compound growth factors include 1% DMEM 2ml, 0.5% DNA 1ml and 1% cystine 2ml; the antibiotic for Candida culture medium is chloramphenicol, the antibiotic for Haemophilus culture medium is vancomycin 1ml and polymyxin 4ml , Mycoplasma medium antibiotics fluconazole 2ml and penicillin 3ml.

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Abstract

The invention relates to a combined culture reagent box of Candida Albicans, hemophilic bacteria and mycoplasma and application thereof. The reagent box comprises a culture medium and a CO2 generator. The culture medium comprises culture medium of Candida Albicans, culture medium of hemophilic bacteria and culture medium of mycoplasma. The pH value of the culture medium is 6.3 plus or minus 0.5. The invention is used to detect Candida Albicans, hemophilic bacteria and mycoplasma, also evaluate the microecology of the urogenital tract of male and female, diagnose the infection of Candida Albicans, hemophilic bacteria and mycoplasma, and evaluate the curative effect of the bacteria. Compared with the prior art, the combined culture reagent box of Candida Albicans, hemophilic bacteria and mycoplasma of the invention has the advantages of the simple operation, the reliable method, the stable characteristics, and easily observing and judging the results, provides a diagnostic tool with more scientific, more convenient, more humanized and more accurate, is favorable for biological or medical practice and commercialized transportation and has the good social and economic efficiency.

Description

technical field [0001] The invention relates to a kit for biomedicine, in particular to a kit for the combined cultivation of candida, haemophilus and mycoplasma and its application. Background technique [0002] Candida, Haemophilus and Mycoplasma are common pathogenic microorganisms in the human genitourinary tract that endanger human health, mainly causing urethritis, vaginitis, prostatitis, cervicitis, pelvic inflammatory disease, infertility, neonatal infection and other diseases. [0003] At present, the clinical detection of Candida, Haemophilus and Mycoplasma mainly adopts medium culture. This culture method has certain defects in terms of sensitivity, specificity, stability, etc., which will have a negative impact on the accuracy of the judgment of the final test result. Contents of the invention [0004] The object of the present invention is to provide a combination culture kit for candida, haemophilus and mycoplasma that is easy to operate, reliable in method,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 叶鹰李书君杨敬芳
Owner 叶鹰
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