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Process for producing 2,3-butanediol using american artichoke as raw material by fermentation

A technology of butanediol and Jerusalem artichoke, which is applied in the fields of fermentation production and microbial fermentation production, can solve the problems of restricting large-scale production and wide application and influence, and achieve the effects of saving fermentation time, wide planting range and increasing concentration

Inactive Publication Date: 2012-05-23
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although 2,3-BD has such a wide application potential, its high price has always affected and limited its large-scale production and wide application
People such as Jacques Fages have also tried to produce 2,3-BD (Appl Microbiol Biotechnol, 1986, 25 (3): 197-202) with Jerusalem artichoke as a raw material by batch fermentation, but obtained under optimal culture conditions The concentration of 2,3-BD is only 44g / L

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Using Jerusalem artichoke juice as raw material, adopt step-by-step saccharification and fermentation process:

[0021] Wash the Jerusalem artichoke tubers, press them, concentrate and adjust the paste to obtain the Jerusalem artichoke juice, add 30U / g of Jerusalem artichoke dry weight inulinase to the Jerusalem artichoke thick juice at 55°C and pH 6.0, and hydrolyze it for 12 hours to obtain a reducing sugar concentration of 254.2 g / L inulin hydrolyzate; sterilize 1L inulin hydrolyzate separately at 115°C for 15min, and mix it with 500mL sterilized fermentation medium after cooling; inoculate the seeds cultivated for 22 hours with 5% The fermentation culture was carried out under the conditions of 37°C, 300rpm, pH6.0, and aeration rate of 0.1vvm; the culture method adopts batch fed-batch fermentation, and when the concentration of residual reducing sugar is lower than 50g / L, additional 200ml of inulin hydrolyzate with a reducing sugar concentration of 368g / L was fermen...

Embodiment 2

[0023] Using Jerusalem artichoke powder as raw material, adopt step-by-step saccharification and fermentation process:

[0024] For the convenience of storage, wash, dry and crush Jerusalem artichoke tubers to make Jerusalem artichoke powder, add water to make a slurry before use; add 400g of Jerusalem artichoke powder to water to make a slurry and set the volume to 1L, and add 30U / g Jerusalem artichoke dry weight inulinase, hydrolyzed for 12 hours to obtain an inulin hydrolyzate with a reducing sugar concentration of 246.50g / L; sterilize it separately at 115°C for 15min, and ferment it with 500mL sterilized fermentation culture after cooling The inorganic salt components in the base were mixed; the seeds cultivated for 22 hours were inoculated according to the inoculation amount of 5%, and the fermentation culture was carried out at 37°C, 300rpm, pH6.0, and the ventilation rate was 0.1vvm; the cultivation method was batch fermentation. method, the fermentation was carried ou...

Embodiment 3

[0026] Using Jerusalem artichoke powder as raw material to adopt simultaneous saccharification and fermentation process:

[0027] Add water to 400g of Jerusalem artichoke powder to make slurry to 1L, sterilize it separately at 115°C for 15min, and mix it with 500mL of sterilized fermentation medium after cooling; mix the seeds cultivated for 22 hours with 5% The inoculum amount was inserted, and at the same time, the glucoamylase that had been sterilized by the membrane was added. The amount of glucoamylase added was 30U / g dry weight of Jerusalem artichoke; the fermentation culture was carried out at 37°C, 300rpm, pH6.0, and the ventilation rate was 0.1vvm The culture method adopts the mode of batch fermentation; when fermenting for 24 hours, the concentration of 2,3-BD in the fermentation broth is 48.95g / L, and the sum of the concentration of 2,3-BD and acetoin has reached 53.91g / L, the concentration of 2,3-BD was 70.11g / L during 40h of fermentation, the sum of the concentrat...

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PUM

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Abstract

The invention discloses a method of producing 2, 3-butanediol by fermenting with Jerusalem artichoke as raw material, which belongs to the biochemical technology field. The method is characterized in that preparing raw material of Jerusalem artichoke tuber to Jerusalem artichoke juice or pulp, and adding inulase in the Jerusalem artichoke juice or pulp to obtain inulin hydrolysate. Using the Jerusalem artichoke juice, the pulp or the innulin hydrolysate as a carbon source respectively, mixing the Jerusalem artichoke juice, the pulp or the innulin hydrolysate with nutrient content of sterilized inorganic salt, accessing to a strain producing the 2, 3-butanediol and obtaining higher concentration of the 2, 3-butanediol through fractional diastatic fermentation and synchronous diastatic fermentation. The method has the advantages that the raw material cost to produce the 2,3-butanediol by microbial fermentation can be reduced, the production efficiency can be improved, the ultimate density of the 2, 3-BD can be between forty-nine and one hundred and five g / L; the ultimate concentration of 49-105g / L of the 2, 3-BD can be obtained; and the final concentration sum of the 2, 3-BD and acetoin is between 60 and 115g / L.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a method for fermenting and producing 2,3-butanediol, in particular to a method for producing 2,3-butanediol by using Jerusalem artichoke as a raw material through microbial fermentation. Background technique [0002] 2,3-Butanediol (English name: 2,3-Butanediol, hereinafter referred to as 2,3-BD) is an extremely important chemical raw material with a wide range of applications. It is an extremely valuable liquid fuel; and it is easy to dehydrate to obtain important industrial organic solvents or fuel additives, methyl ethyl ketone, and monomers -2-butene and 1,3-butadiene for synthetic plastics and artificial rubber; while 2,3- BD is also easy to catalyze dehydrogenation to obtain acetoin and diacetyl compounds, which are used to make high-value spices and food additives; the esterified form of 2,3-BD is a precursor for the synthesis of polyimine, which can be used in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12N1/20C12R1/01C12R1/12C12R1/22
Inventor 修志龙孙丽慧王旭东孙亚琴戴建英
Owner DALIAN UNIV OF TECH
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