Method for producing sorbitol and gluconate

A technology of gluconate and gluconic acid, applied in the field of producing sorbitol and gluconate, can solve the problem of high cost of sorbitol, and achieve the effects of low price, reduction of by-product generation, and cost reduction

Inactive Publication Date: 2013-09-11
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies in the prior art, provide a kind of technical method that uses non-grain cheap raw material Jerusalem artichoke to replace fructose and glucose to produce sorbitol, solve the problem that the cost of producing sorbitol mainly using sugar as a substrate is relatively high at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Using inulin as raw material, add H at 70°C 2 SO 4 , so that H 2 SO 4The final concentration is 1%, hydrolyzed for 5 hours, the concentration of reducing sugar glucose and fructose obtained is respectively 26g / L and 132g / L inulin hydrolyzate, centrifuge the hydrolyzate, take out the solid part, the obtained inulin hydrolyzate does not need to be sterilized Directly used in cell catalytic reactions.

[0029] The catalytic reaction is carried out in a fermenter with a total reaction volume of 200mL, including 175mL of inulin hydrolyzate and 25mL of glucose solution, so that the concentration of glucose and fructose in the reaction system is about 91.1g / L, and then 60g / L is added to cultivate Centrifuged wet cell seeds, the middle temperature of the reactor was raised to 40° C., the rotation speed was 150 rpm, the final amount of sorbitol was 86.37 g / L, the yield was 0.937 (g / g), and the conversion rate was 0.937.

Embodiment 2

[0031] Wash 600g of fresh Jerusalem artichoke with purple skin, add H 2 SO 4 solution to a solids content of 15% H 2 SO 4 The concentration is 0.1M, and the hydrolysis reaction is carried out in the reactor at 70°C. The obtained hydrolyzate is centrifuged to collect the supernatant, and the supernatant is used as a raw material to catalyze the catalytic reaction to generate sorbitol and gluconic acid. Add glucose in the reactor to make glucose and fructose reach equal mass, the total volume of reaction system is 200ml, the temperature of reactor is raised to 40 ℃, rotating speed 150rpm, then add seed cell and suspend 12g (wet weight) cell, finally The reaction system contains equal mass of glucose and fructose which is 68g / L, the final amount of sorbitol is 40g / L, the yield is 0.59(g / g), the conversion rate is 100%, and the concentration of ethanol is 43g / L.

Embodiment 3

[0033] The Jerusalem artichoke hydrolyzate is concentrated through the column to obtain Jerusalem artichoke high fructose syrup. The Jerusalem artichoke high fructose syrup is used as the raw material. The catalytic reaction is carried out in a fermenter with a total reaction volume of 200mL. Seed cells suspend 12g (wet weight) cells, add in the fermenter, the final reaction system contains equal quality glucose and fructose is 500g / L, the amount of final sorbitol is 194.7g / L, and the productive rate is 0.80(g / L g), the conversion rate is 0.947, and the concentration of ethanol is 16.3g / L.

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Abstract

The invention relates to a method for producing sorbitol and gluconate, which comprises the following steps of: preparing jerusalem artichoke tubers serving as a raw material into jerusalem artichoke juice or jerusalem artichoke pulp, and adding inulase or diluted acid into the jerusalem artichoke juice or pulp to obtain jerusalem artichoke hydrolysis solution rich in fructose and glucose monosaccharide; supplementing a proper amount of glucose by using the jerusalem artichoke hydrolysis solution as a carbon source till the mass or the mole content of the glucose and the fructose is the same; and regulating pH, temperature, reaction time, mixing mode and strength, cell concentration and cell catalysis mode (free or immobilized) of the biological reaction system by using wild or transgenic Zymomonas mobilis cells as a biological catalyst, and converting the jerusalem artichoke hydrolysis solution into the sorbitol and the gluconate. The method can also be applied in fermentation of carbohydrate raw materials such as jerusalem artichoke hydrolysis syrup, cane molasses, sweet sorghum juice and the like or cell catalysis for preparing the sorbitol and the gluconate. The method has the advantages that: raw material planting range is wide, the cost of producing the sorbitol by a biological method is reduced, and gluconic acid is obtained.

Description

【Technical field】 [0001] The invention relates to a method for producing sorbitol and gluconate by using Jerusalem artichoke as raw material through microbial catalytic reaction. 【Background technique】 [0002] As an important chemical product, sorbitol is widely used in medicine, food, light chemicals, surfactants, synthetic resins, etc. It is a green product that can meet various applications (Vogel, 2002). Sorbitol is one of the 12 key intermediates with the most development potential evaluated by the National Renewable Energy Laboratory (NREL) and the Pacific Northwest National Laboratory (PNNL). Using sorbitol as a biorefinery intermediate, a variety of important materials and chemicals that can replace petrochemicals can be produced. The main derivatives include isosorbide, propylene glycol, polysorbate fatty acid ester ( sorbitan fatty acid esters, SFAE), lactic acid, glycerol, polysaccharides (branched polysaccharides), etc., all of which have important industrial v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12P7/58C12R1/01
Inventor 鲍杰胡凤仙刘长俊
Owner EAST CHINA UNIV OF SCI & TECH
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