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Method for preserving Porphyra haitanensis young seedling at low-temperature

A preservation method and technology of laver, applied in the preservation of plants, botanical equipment and methods, applications, etc., can solve problems such as no reports that have not been seen, and achieve the effect of improving survival rate and saving workload

Inactive Publication Date: 2008-12-24
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the fresh and alive cold storage of laver fronds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] Assuming that during genetic breeding of Porphyra haitanensis, a large number of Porphyra haitanensis seedlings were obtained through the release and germination of the shell spores of Porphyra haitanensis. The method can be used to cool the fresh seedlings of Porphyra haitanensis at low temperature. Specific steps are as follows:

[0017] 1) Prepare refrigeration protective agent with disinfected seawater with a salinity of 25‰, which contains 10% glycerin, 5% DMSO, 15% glucose, and 70% disinfected seawater by volume percentage;

[0018] 2) Take 30ml of the prepared refrigeration protective agent, and divide it into 50ml cryo-storage tubes;

[0019] 3) Place the fresh seedlings of Porphyra haitanensis with a size of 1~3cm directly in the cryo-storage tube containing the refrigeration protective agent;

[0020] 4) After sealing the cryopreservation tube, store it in a refrigerator at -20~-25℃.

[0021] After storing for a period of time, when you need to use these seedlings...

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PUM

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Abstract

The invention discloses a method for deep-freezing of Porphyra haihanensis seedlings, relating to Porphyra haihanensis, particularly relating to a method for deep-freezing of Porphyra haihanensis seedlings in the aquiculture field. A proper amount of refrigeration protectant is prepared; the refrigeration protectant is separately filled in a freezing storage tube; Porphyra haihanensis seedlings are collected; the collected Porphyra haihanensis seedlings are placed in the freezing storage tube filled with refrigeration protectant; and the freezing storage tube filled with refrigeration protectant is kept under cold storage. When resuscitation is required, the freezing storage tube filled with refrigeration protectant is placed in water for thawing and then taken out. The resuscitated Porphyra haihanensis seedlings kept under deep-freezing 180d can still have a survival rate above 95 percent after recovery cultivation. Because refrigeration protectant is used to preserve fresh Porphyra haihanensis seedlings, the processes of thallus separation, drying, cooling, etc. are required by conventional low-temperature preservation; thereby, the labor is saved to large extent, and the survival rate of Porphyra haihanensis thallus preserved under lower temperature is improved.

Description

Technical field [0001] The invention relates to a laver, in particular to a low-temperature cold storage method for seedlings of laver in the field of aquaculture. Background technique [0002] Porphyra haitanensis (Porphyra haitanensis) is a unique warm-temperate variety in my country. It is an important economic red algae cultivated in the coastal areas of Fujian, Zhejiang and Guangdong, and its output accounts for about 80% of the total national output of laver. [0003] There have been a lot of researches on the low-temperature storage of Porphyra yezoensis species. At present, the fronds of Porphyra yezoensis are generally dried in the shade and then stored in the refrigerator at -20~-30℃. However, the method of Porphyra genetics In breeding research, it is often necessary to process the sprouts or seedlings after each experiment in large batches. Since the seedlings are relatively small and fragile, the separation and drying in the shade before freezing is troublesome and r...

Claims

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Application Information

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IPC IPC(8): A01N3/00
Inventor 陈昌生谢潮添纪德华徐燕
Owner JIMEI UNIV