T2r taste receptors and genes encoding same
A taste receptor and encoding technology, applied in the field of genes and cDNA, can solve the problems of lack of understanding of molecules and pathways that mediate sensory signal responses
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[0252] Preparation and screening of combinatorial chemical libraries is well known to those skilled in the art. Such combinatorial chemical libraries include, but are not limited to, peptide libraries (see, e.g., U.S. Patent 5,010,175, Furka, Int. J. Pept. Prot. Res., 37:487-493 (1991) and Houghton et al., Nature , 354:84-88 (1991)). Other chemistries for generating chemically diverse libraries can also be used. These chemicals include, but are not limited to: peptoids (eg WO91 / 19735), encoded peptides (eg WO 93 / 20242), random bio-oligomers (PCT eg WO 92 / 00091), benzodiazepines (e.g. U.S. Pat. No. 5,288,514), hydantoins and benzodiazepines And dipeptide heteropolymers (diversomers) (Hubbs et al., PNAS90: 6906-6913 (1993)), ethylene (vinylogous) polypeptides (Hagihara et al., J.Amer.Chem. Soc., 114: 6568 (1992)) , nonpeptidal petidomimetics with a glucose scaffold (Hirschmann et al., J.Amer.Chem.Soc., 114:9217-9218 (1992)), similar organically synthesized small molecule co...
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[0267] The following examples summarize the isolated nucleic acid molecules of the present invention, and the corresponding polypeptide sequences to conceptual translations of the nucleic acid molecules. In the protein sequences provided here, the single letter X or Xaa refers to any one of the twenty common amino acid residues. In the DNA sequences provided here, the single letter N or n represents any of the four common nucleotide bases A, T, C, and G.
[0268] hT2R51 full-length cDNA (BAC AC011654) (SEQ ID NO: 1)
[0269] ATGTTGACTCTAACTCGCATCCGCACTGTGTCCTATGAAGTCAGGAGTACATTTCTGTTCA
[0270] TTTCAGTCCTGGAGTTTGCAGTGGGGTTTCTGACCAATGCCTTCGTTTTCTTGGTGAATTTT
[0271] TGGGATGTAGTGAAGAGGCAGGCACTGAGCAACAGTGATTGTGTGCTGCTGTGTCTCAGC
[0272] ATCAGCCGGCTTTTCCTGCATGGACTGCTGTTCCTGAGTGCTATCCAGCTTACCCCACTTCCA
[0273] GAAGTTGAGTGAACCACTGAACCACAGCTACCAAAGCCATCATCATGCTATGGATGATTGCA
[0274] AACCAAGCCAACCTCTGGCTTGCTGCCTGCCTCAGCCTGCTTTACTGCTCCAAGCTCATCC
[0275] GTTTCTCTCACACCTTCCTGATCTG...
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