Unlock instant, AI-driven research and patent intelligence for your innovation.

High throughput five-nest type PCR transgenic detection method for finely processed product of food crop

A detection method and technology for food crops, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of lack of DNA extraction technology, lack of serialized technology, single type of extraction kit, etc., to improve detection. The effect of reducing the throughput, reducing the detection cost, and reducing the detection time

Inactive Publication Date: 2009-04-08
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The DNA extraction technology required for genetically modified detection of deep-processed products is still in the research stage abroad, and has not formed a serialized technology, and the commercialized extraction kits are of a single type
Due to the wide variety of deep-processed products and complex

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High throughput five-nest type PCR transgenic detection method for finely processed product of food crop
  • High throughput five-nest type PCR transgenic detection method for finely processed product of food crop
  • High throughput five-nest type PCR transgenic detection method for finely processed product of food crop

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0048] The present invention will be further described below in conjunction with specific embodiment:

[0049] 1. Experimental materials

[0050] (1) Positive standard products: transgenic soybeans, corn, rice;

[0051] Negative standards: non-GMO soybeans, corn, rice;

[0052] Samples to be inspected: deep-processed soybean protein powder, chocolate drinks, baby rice noodles, soybean lecithin, soybean oil, soybean salad oil, corn oil, corn starch, corn gluten powder, nutritional oatmeal, corn puree;

[0053] (2) Reagents

[0054] Magnetic DNA Purification System for Food kit; r-Taq DNA polymerase, dNTP, 10×PCR buffer (containing MgCl2) and DL-2000 molecular mass standard.

[0055] 2. Main instruments and equipment

[0056] High-speed centrifuge (Shanghai Anting, TDL-40B), PCR instrument (Germany Biometra, Biometra Tgradient), nucleic acid electrophoresis instrument (Hangzhou Dahe, GE-100), UV spectrophotometer (USA BECKMAN, 640), gel imaging system (U.S. UVP, G8000). ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a transgenic detection method of 5-plex nest PCR which is the deeply processed product of grain crops, comprising the following detection steps: 1. DNA extraction and concentration detection; 2. primer design; 3. reaction systems and conditions of the first and second round of 5-plex nest PCR; 4. the detection of amplified products; and 5. result determination. The technique of the invention has a plurality of advantages of being high in flux, rapid, accurate, sensitive and economical, can be used for detecting not only the sing deeply-processed products of transgenic soybean, maize and rice, but also the mixed deeply-processed products of the transgenic soybean, maize and rice. The method improves the detection efficiency, reduces the detection time and cost and can effectively decrease the results of false positive. Therefore, the method can be applied to the DNA detection of transgenic foods deeply processed from grain crops such as soybeans, maizes, rice and bean oil and the like and has practical meaning.

Description

(1) Technical field [0001] The invention is a high-throughput transgene detection method for deep-processing products of major food crops. (2) Technical background [0002] With the establishment and continuous improvement of genetically modified organism (GMO) labeling laws in various countries, accurate detection of genetically modified ingredients is becoming increasingly important. Many countries not only require qualitative testing of genetically modified Quantitative detection for identification. The European Union and other countries and regions have successively introduced a labeling system for genetically modified products, and imported and exported products must issue a test report on whether they contain genetically modified ingredients. In recent years, my country has imported a large amount of genetically modified soybeans for processing. Due to insufficient market identification, the export trade of my country's processed products has suffered losses. In orde...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 敖金霞高学军曲波李庆章刘营袁肖寒仇有文
Owner NORTHEAST AGRICULTURAL UNIVERSITY