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Effective hypoglycemic component of may flower and preparation method and use thereof

An effective part and a hypoglycemic technology, which is applied to the effective part of osmanthus fragrans for hypoglycemic and its preparation and application fields, can solve the problems of unreported medicinal value of osmanthus fragrans, achieve low cost, inhibit the activity of α-glucosidase, and have wide distribution. Effect

Inactive Publication Date: 2009-04-15
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Osmanthus fragrans is mainly used in the production of traditional osmanthus fragrans food and high-grade spices, but the application of osmanthus fragrans medicinal value has not been reported yet

Method used

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  • Effective hypoglycemic component of may flower and preparation method and use thereof
  • Effective hypoglycemic component of may flower and preparation method and use thereof
  • Effective hypoglycemic component of may flower and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This example is the extraction method and preliminary activity screening of each effective part of sweet-scented osmanthus

[0023] The extraction method of the effective part is as follows: take the dried golden osmanthus (1.9kg), pulverize it, soak it coldly with acetone and water (7:3 by volume) / alcohol for 3 times, each time for 7 days, filter, combine the filtrate, and depressurize The solvent was recovered to obtain a concentrate; the concentrated extract was dispersed in water, and extracted three times with petroleum ether, ethyl acetate, and n-butanol in sequence to obtain 10 g, 33.4 g, and 400 g of the corresponding extracts. The alcohol used for extraction in this embodiment is preferably ethanol.

[0024] Activity assay:

[0025] Method: Microplate method

[0026] Principle: α-D-glucosidase catalyzes the hydrolysis of 4-nitrobenzene-α-D-glucopyranoside (PNP-G) to produce nitrophenol (PNP, a yellow substance with maximum absorption at around 400m), α -Glyc...

Embodiment 2

[0037] This example is a method for preparing the active compound of hypoglycemic active ingredient from the active part of osmanthus osmanthus ethyl acetate under the guidance of activity

[0038] The preparation method of the hypoglycemic active ingredient is specifically as follows: 33.4 g of the ethyl acetate part is subjected to 200-300 mesh silica gel column chromatography, and gradient elution is carried out with chloroform:methanol (100:1→8:2), to obtain The part of glycosidase activity; this part is through silica gel H column chromatography, sherwood oil-acetone (20: 1) elution, detects and merges with TLC, through Sephadex LH-20 column chromatography, acetone elution, obtains compound 1 (100.2mg ), 2 (11.2 mg).

Embodiment 3

[0040] This example is the determination of the activity of the compound obtained from Osmanthus fragrans to inhibit α-glucosidase.

[0041] Measuring method: with embodiment 1.

[0042] Measuring results: the experimental results are shown in Table 2. The inhibitory activity of ursolic acid and oleanolic acid on α-glucosidase was as high as 107.0%, and the half inhibitory rate was only 0.003 μg·m L -1 , which is much better than the α-glucosidase inhibitory activity of acarbose, a commonly used hypoglycemic drug in clinical practice, and can be developed as the main medicinal ingredient of sweet-scented osmanthus fragrans for inhibiting α-glucosidase activity. The relationship between the hypoglycemic activity and the chemical structure of the active components can be further discussed in detail, which plays an important role in the discovery of efficient and safe new drugs for reducing postprandial blood sugar.

[0043] The α-glucosidase inhibitory activity of compound in ...

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Abstract

The invention discloses a hypoglycemic effective position for sweet osmanthus, a preparation method and application thereof. The effective position is an ethyl acetate position and / or a normal butanol position which is obtained by taking sweet osmanthus as a raw material, and after extraction of condensate by crushing, cold soaking, filtration and vacuum recovery. The hypoglycemic effective position, namely ethyl acetate extract, has obvious activity in inhibiting alpha-glucosaccharase; and hypoglycemic effective constituents obtained from the effective position, namely an ursolic acid and an oleanolic acid, also have high activity in inhibiting the alpha-glucosaccharase, wherein the IC50 value (the half inhibition ratio) is 0.003Mug.m L <-1>, and the activity of the ursolic acid and the oleanolic acid in inhibiting the alpha-glucosaccharase is better than that of a hypoglycemic medicine - acarbose (the IC50 value is 1,081.27 Mug.m L <-1>) which is commonly used on clinic under the same condition.

Description

technical field [0001] The present invention relates to an effective part of osmanthus fragrans for lowering blood sugar and a preparation method thereof, an active ingredient extracted from the effective part and a preparation method thereof, and meanwhile, application of the effective part and the effective ingredient. Background technique [0002] Diabetes mellitus is a systemic disease characterized by hyperglycemia, metabolic disorders, and closely related to insulin secretion. It is a clinical syndrome caused by the interaction of genetic and environmental factors. Absolute or relative insufficiency of insulin secretion and decreased sensitivity of target tissues to insulin lead to a series of metabolic disorders of sugar, protein, fat, water and electrolytes. Diabetes is mainly divided into two types: type I diabetes (ie, insulin-dependent diabetes) and type II diabetes (ie, non-insulin-dependent diabetes), the latter accounting for about 99%-95% of the total number o...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K31/56A61P3/10C07J63/00A61K133/00
Inventor 康文艺宋艳丽王金梅
Owner HENAN UNIVERSITY
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