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Preparation method of inonotus obilquus as alpha-glucosidase inhibitors

A technology of glucosidase and Inonotus obliquus, which is applied in the field of preparation of Inonotus obliquus extracts, can solve problems such as the extraction of α-glucosidase inhibitors that have not yet been found, and achieve strong inhibitory activity, simple process and convenient operation. Effect

Inactive Publication Date: 2009-05-06
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no method for extracting α-glucosidase inhibitors from Inonotus obliquus has been found.

Method used

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  • Preparation method of inonotus obilquus as alpha-glucosidase inhibitors

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) 100g of Inonotus obliquus is crushed and passed through a 30-mesh sieve, then 1000mL of 80% ethanol is used as the extraction solvent, heated and refluxed at 70°C for 3 times for 1 hour each time, the extracts are combined, and centrifuged supernatant, and the supernatant was concentrated and dried to obtain 16.2g ethanol extract;

[0018] (2) Disperse the above-mentioned ethanol extract in 324mL distilled water, then use 162mL petroleum ether to vibrate and extract 3 times for 2 hours each time, and separate the extract from the aqueous phase raffinate;

[0019] (3) The above aqueous phase raffinate was shaken and extracted with 162mL ethyl acetate for 3 times, each time for 2 hours, and then the extracts were combined, concentrated and dried to obtain 2.1g of α-glucosidase with strong inhibitory activity of tan powdered Inonotus obliquus extract.

Embodiment 2

[0021] (1) 100g of Inonotus obliquus is crushed and passed through a 30-mesh sieve, then 1200mL of 80% ethanol is used as the extraction solvent, heated and refluxed at a temperature of 75°C for 3 times, each time for 2 hours, the extracts are combined, and suction filtered , separate the supernatant, and obtain 18.1g ethanol extract after the supernatant is concentrated and dried;

[0022] (2) Disperse the above-mentioned ethanol extract in 543mL of distilled water, then shake and extract with 362mL petroleum ether for 4 times, each time for 2 hours, and separate the extract from the aqueous phase raffinate;

[0023] (3) The above-mentioned aqueous phase raffinate was extracted by shaking with 362mL ethyl acetate 4 times, each time for 2 hours, and then the extracts were combined, concentrated and dried to obtain 3.3g of α-glucosidase with strong inhibitory activity of tan powdered Inonotus obliquus extract.

Embodiment 3

[0025] (1) After crushing 100g of Inonotus obliquus, pass through a 30-mesh sieve, then use 1200mL of 80% ethanol as the extraction solvent, heat and reflux at a temperature of 75°C for 5 times for 2 hours each time, combine the extracts, and suction filter , separate the supernatant, and obtain 22.3g ethanol extract after the supernatant is concentrated and dried;

[0026] (2) Disperse the above-mentioned ethanol extract in 1115mL of distilled water, then use 669mL of petroleum ether to vibrate and extract 4 times, each time for 4 hours, and separate the extract from the aqueous phase raffinate;

[0027] (3) The above-mentioned aqueous phase raffinate was extracted by shaking with 669mL ethyl acetate 4 times, each time for 4 hours, and then the extracts were combined, concentrated and dried to obtain 4.0g of α-glucosidase with strong inhibitory activity of tan powdered Inonotus obliquus extract.

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Abstract

The invention discloses a preparation method of an Inonotus obliquus extract which can be used as an Alpha-glucosaccharase inhibitor. In the preparation method, ethanol is firstly adopted to heat and reflow the Inonotus obliquus so as to obtain an ethanol extract, then the ethanol extract is fully dispersed into distilled water and is extracted by sherwood oil so as to remove low-polarity components in the extract, which have no inhibition activity to the Alpha-glucosaccharase; and then the raffinate is extracted by ethyl acetate, thereby obtaining the extract which has strong inhibition activity to the Alpha-glucosaccharase; and the yield rate is 2 to 4 percent. Experiment proves that the Inonotus obliquus extract obtained by the preparation method provided by the invention has very strong inhibition activity to the Alpha-glucosaccharase; when the dosage is 400 Mug, the inhibition rate can reach 80.5 percent, with the IC50 of 103.9 Mug / ml; therefore, the method can be used for developing sugar-reducing medicament. Moreover, the preparation method also has the advantages of simple technique, convenient operation, and the like.

Description

technical field [0001] The invention relates to a method for preparing an Inonotus obliquus extract which can be used as an α-glucosidase inhibitor, and belongs to the technical fields of preparation of active ingredients of Chinese herbal medicines and chemical engineering. Background technique [0002] Diabetes mellitus is a metabolic disease of multiple etiologies characterized by chronic hyperglycemia accompanied by disturbances in glucose, fat and protein metabolism due to defects in insulin secretion and action. At present, the prevalence of diabetes in developed countries is above 6-10%. The number of patients in my country is more than 40 million, and the prevalence is about 2-4%. It has become the third "health killer" after cardiovascular and tumors, especially diabetes. As the global population ages, the incidence of the disease continues to rise. Diabetes can lead to blindness, cardiovascular and cerebrovascular diseases, renal failure, neuropathy, limb damage le...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61P3/10
Inventor 陈海霞卢雪明
Owner TIANJIN UNIV
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