DLK1 gene nucleic acid hybridization in situ detection kit, detection method and uses thereof

Abstract

The invention relates to a kit for detecting hybridization in situ for DLK1 gene of early cancer, which comprises a hybridization probe and a marker, wherein the sequence of the hybridization probe is expressed as SEQ ID NO.1. The invention also provides a method for detecting hybridization in situ for the DLK1 gene. The detection kit and the detection method can detect expression amount of the DLK1 gene in early canceration cells or in canceration cells at the level of genes, can detect change information of the DLK1 gene before generation of protein markers and more before oncogenesis, and really perform early diagnosis of tumor. The kit has the advantages of high sensitivity and strong specificity. The detection method has the advantage of convenient and simple operation, and can be generally used and popularized in hospitals above districts.

Description

technical field

[0001] The invention relates to the field of biological detection and disease diagnosis, and more specifically relates to the gene detection technology of cancer. Background technique

[0002] According to the information provided by authoritative organizations at home and abroad, there are 1.6 million new cancer cases in my country every year, nearly 1.6 million deaths, and 6 million patients. Globally, there are 8 million new cancer patients every year, nearly 8 million deaths, and about 84 million patients. , and the number will double by 2020. The incidence and location of male cancers are arranged in order of lung, liver, and stomach; in females, they are arranged in order of breast cancer, cervical cancer, and ovarian cancer. The above cancers account for more than half of the total cancer cases.

[0003] From the onset of cancer to the survival period after treatment, taking liver cancer as an example, it is difficult to break through the five-year sur...

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