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Kit for LAMIN A gene hybridization in situ, detection method and use thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve problems such as the unreported LAMINA gene detection technology and detection kits, imperfect design ideas of anticancer drugs, drug resistance of tumor cells, etc., and achieve a radical cure Malignant bowel cancer, high sensitivity, strong specificity

Active Publication Date: 2011-09-14
台州和和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war are: 1. Heterogeneity of tumor cells; 2. Drug resistance of tumor cells; 3. Incomplete design of anticancer drugs, etc.
According to the existing literature and novelty search reports, there is no report on the detection technology and detection kit of LAMIN A gene

Method used

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  • Kit for LAMIN A gene hybridization in situ, detection method and use thereof
  • Kit for LAMIN A gene hybridization in situ, detection method and use thereof
  • Kit for LAMIN A gene hybridization in situ, detection method and use thereof

Examples

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Effect test

Embodiment 1

[0051] An in situ hybridization detection kit for LAMIN A gene, comprising a hybridization probe, a marker, and a synergist, wherein the sequence of the hybridization probe is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0052] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0053] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0054] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0055] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0056] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0057] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0058] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0059] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0060] ...

Embodiment 2

[0102] A kind of LAMIN A gene in situ hybridization detection method and its kit application

[0103] 1. Specimen processing

[0104] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10 minutes;

[0105] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10 min;

[0106] 3. Discard the supernatant. Add approximately twice the amount of 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10 min;

[0107] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry natural...

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Abstract

The invention relates to a reagent kit for detecting in situ hybridization of a LAMIN A gene, which comprises a hybridization probe and a marker, wherein the sequence of the hybridization probe is shown as SEQ ID NO.1. The invention also provides a LAMIN A gene in situ hybridization detecting method. In addition, the invention also provides application of the reagent kit to the preparing and detecting of medicines for intestinal cancer. The reagent kit has the characteristics of high sensitivity and strong specificity. The detecting method has the advantages of simple and convenient operation, and wide application and popularization in hospitals above district level.

Description

【Technical field】 [0001] The present invention relates to a kit, in particular to a LAMIN A gene in situ hybridization detection kit and its detection method and application. 【Background technique】 [0002] According to the information provided by authoritative organizations at home and abroad, there are 1.6 million new cancer cases in my country every year, nearly 1.6 million deaths, and 7 million patients. Globally, there are 8 million new cancer patients every year, nearly 8 million deaths, and about 84 million patients. , by 2020 the number of people will double, which is a set of terrible figures. Colon cancer is one of the most common malignant tumors. Among digestive tract tumors, the incidence of bowel cancer is getting higher and higher, and the rate of bowel cancer among young people is increasing year by year. The incidence of colorectal cancer in Shanghai ranks sixth among all malignant tumors, and now it has jumped to the second place. Among them, colon cancer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 裘建英张云福
Owner 台州和和生物科技有限公司
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