Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for NKX2-8 gene hybridization in situ, detection method and application thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve the problems of imperfect design ideas of anticancer drugs, unreported detection technology, drug resistance of tumor cells, etc. specific effect

Active Publication Date: 2011-11-23
台州和和生物科技有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]In 2005, the American Institute of Health, the Cancer Institute, the Centers for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer" , that is to say, the cancer mortality rate has not been reduced, and it lists several factors that lead to the failure of the anti-cancer war: 1. Tumor cell heterogeneity; 2. Tumor cell drug resistance; 3. Incomplete design of anticancer drugs, etc.
However, there is no report about the in situ hybridization detection kit and detection technology of NKX2-8 gene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for NKX2-8 gene hybridization in situ, detection method and application thereof
  • Kit for NKX2-8 gene hybridization in situ, detection method and application thereof
  • Kit for NKX2-8 gene hybridization in situ, detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] An in situ hybridization detection kit for NKX2-8 gene, comprising hybridization probes, markers and synergists, wherein the sequence of the hybridization probe is completely reverse complementary to the sequence shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0047] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0048] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0049] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0050] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0051] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0052] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0053] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0054] Chromogen A 17...

Embodiment 2

[0097] A kind of NKX2-8 gene in situ hybridization detection method and its kit application

[0098] 1. Specimen processing

[0099] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0100] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0101] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0102] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with condi...

Embodiment 3

[0137] A comparative experiment between the detection of lung cancer disease with the NKX2-8 gene kit and the detection of lung cancer disease with the TIG2 gene kit.

[0138] In order to scientifically evaluate the specificity, sensitivity and accuracy of the above genes in lung cancer. We use the parallel test method to detect the mRNA of the above genes at the same time. The detection technology adopts nucleic acid in situ hybridization technology, and uses the peripheral blood of the same patient with lung cancer to simultaneously detect the mRNA of NKX2-8 gene and TIG2 gene (nucleic acid in situ hybridization , immunohistochemical staining, microscopic counting, result reporting, etc. all adopt the same method and steps and reagents as the in situ hybridization technique of Example 1 and Example 2). It is found that the expression level of NKX2-8 gene in patients with lung cancer is higher than that of TIG2 gene in patients with the same disease. The results showed that ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a kit for detecting in situ hybridization of NKX2-8 gene, comprising a hybridization probe and a label, and the sequence of the hybridization probe is reverse complement as shown in SEQ ID NO.1. The invention also provides a method for detecting in situ hybridization of the NKX2-8 gene. In addition, the invention provides the application of the kit in the preparation of medicine for detecting lung cancer and lung cancer metastases diseases. The kit provided by the invention has the characteristics of high sensitivity and better particularity. The detecting method of the invention is convenient and simple in operation, and can be generally used and popularized in the hospitals which are above county level.

Description

【Technical field】 [0001] The invention relates to a reagent kit, in particular to an in situ hybridization detection reagent kit for NKX2-8 gene and its detection method and application. 【Background technique】 [0002] According to the information provided by authoritative organizations at home and abroad, there are 1.7 million new cancer cases in my country every year, nearly 1.6 million deaths, and 6 million patients. In the world, there are 8 million new cancer patients every year, nearly 8 million deaths, and about 84 million patients. , by 2020 the number of people will double, which is a set of terrible figures. [0003] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war are: 1. Heterogeneity of tumor cel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 张云福裘建英
Owner 台州和和生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com