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In-situ hybridization detection assay kit and detection method for ABCA5 gene and application of kit

A detection kit and in situ hybridization technology, applied in the field of kits, can solve the problems of low incidence and poor prognosis, and achieve the effects of strong specificity, convenient operation and high sensitivity

Inactive Publication Date: 2011-03-23
NATUREGEN BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the incidence of this disease is not high, the tumor grows rapidly and the prognosis is poor

Method used

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  • In-situ hybridization detection assay kit and detection method for ABCA5 gene and application of kit
  • In-situ hybridization detection assay kit and detection method for ABCA5 gene and application of kit
  • In-situ hybridization detection assay kit and detection method for ABCA5 gene and application of kit

Examples

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Effect test

Embodiment 1

[0046] An in situ hybridization detection kit for ABCA5 gene, comprising a hybridization probe, a marker, and a potentiator, wherein the sequence of the hybridization probe is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0047] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0048] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0049] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0050] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0051] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0052] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0053] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0054] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0055] ...

Embodiment 2

[0097] A kind of ABCA5 gene in situ hybridization detection method and its kit application

[0098] 1. Specimen processing

[0099] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0100] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0101] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0102] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with condit...

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Abstract

The invention relates to an in-situ hybridization detection assay kit for ABCA5 gene, which comprises a hybridization probe and a marker, wherein the sequence of the hybridization probe is shown as SEQ ID NO.1. The invention also provides an in-situ hybridization detection method for the ABCA5 gene. In addition, the invention also provides application of the kit in the preparation of medicaments for detecting prostate sarcoma diseases. The invention has the advantages that: the provided kit has the characteristics of high sensitivity and high specificity; and the detection method is convenient and easy to operate and can be commonly used and popularized in hospitals of district-level or above.

Description

【Technical field】 [0001] The present invention relates to a kit, in particular to an ABCA5 gene in situ hybridization detection kit and its detection method and application 【Background technique】 [0002] Prostate sarcoma is a kind of malignant tumor in the prostate, which is different from prostate cancer. Prostate cancer mainly occurs in the prostate epithelium, while prostate sarcoma occurs in the prostate stroma. [0003] Prostatic sarcoma accounts for 0.1% of prostate malignant tumors. It comes from mesoderm and can form various types of sarcoma after different degrees of differentiation. Common rhabdomyosarcoma, leiomyosarcoma, fibrosarcoma, in addition to lymphosarcoma, myxosarcoma, angiosarcoma, chondrosarcoma, etc., but relatively rare. Rhabdomyosarcoma is more common in children, and leiomyosarcoma is more common in adults. [0004] Prostate sarcoma is a rare malignant tumor of the prostate. It accounts for 2.7% to 7.5% of prostate cancer in my country, and 0.1%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张云福裘建英
Owner NATUREGEN BIOTECH SHANGHAI
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