Bacillus licheniformis 202 and use thereof

A Bacillus licheniformis, antagonism technology, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of blank, weak technology, etc., and achieve the effect of easy preservation, fast reproduction speed and strong inhibitory effect

Inactive Publication Date: 2009-05-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Isolation and screening from banana plants to obtain biocontrol bacterial strains with antagonistic effects on Fusarium wilt of banana is of great significance to the control of Fusarium wilt of banana, but the relevant technology is still weak or even blank

Method used

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  • Bacillus licheniformis 202 and use thereof
  • Bacillus licheniformis 202 and use thereof
  • Bacillus licheniformis 202 and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Plate Antagonism Experiment of Bacillus licheniformis 202 on Banana Fusarium wilt No. 4 Race FOC4

[0025] Insert a FOC4 mycelium block with a diameter of 5mm in the center of the potato sucrose medium PDA plate, and then put a sterilized filter paper sheet with a diameter of 5mm on the triangle of the equidistant FOC4 mycelium block, and insert Bacillus licheniformis on each filter paper sheet 202 culture solution 10μL, take the plate without Bacillus licheniformis 202 culture solution as the control, culture in the incubator at 25-28°C for 5-7 days, measure the diameter of the inhibition zone, with attached figure 1 It can be seen that Bacillus licheniformis 202 has a strong inhibitory effect on Fusarium wilt of banana.

Embodiment 2

[0026] Example 2 Bacillus licheniformis 202 prevents the main root and vascular bundle of banana plants from browning or rotting experiments, and the control effect of greenhouse potted plants.

[0027] Select Brazilian banana seedlings with a plant height of about 15 cm, and carry out 3 different treatments: (1) clean water healthy plant control: the roots of banana seedlings are soaked in sterile water and then directly transplanted in a nutrient bowl to grow; (2) the roots of banana seedlings are soaked in sterile water. After 30 minutes in the culture medium of Bacillus licheniformis 202, transplant it into the nutrient bowl, then pour 20mL of FOC4 spore suspension into the soil of the nutrient bowl; (3) Incidence control: Roots of banana seedlings were inoculated with 20mL of FOC4 spores Suspension, not inoculated with Bacillus licheniformis 202 culture solution. For the above treatments, there were 20 plants in each treatment group. Afterwards, they were cultured at 26°...

Embodiment 3

[0028] Example 3 Antagonism of Bacillus licheniformis 202 to various pathogenic bacteria

[0029] The antagonism of Bacillus licheniformis 202 to 7 pathogenic bacteria including tomato fusarium wilt, Curvularia maize leaf spot, Glue sporotrichum anthracnose, cotton fusarium wilt, cotton Verticillium dahliae, wheat scab and watermelon wilt was tested by plate confrontation method effect, insert a target mycelium block with a diameter of 5mm in the center of the PDA plate, and then put a sterilized filter paper sheet with a diameter of 5mm on the triangle of the equidistant FOC4 mycelium block, and insert Bacillus licheniformis 202 into each filter paper sheet for cultivation 10 μL of solution, and the plate without Bacillus licheniformis 202 culture solution was used as a control, cultured in a 28°C incubator for 5-7 days, and the diameter of the inhibition zone was measured. Bacillus licheniformis 202 has a wide antibacterial spectrum and has antagonistic effects on various pa...

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Abstract

The invention discloses Bacillus licheniformis 202, which has been preserved in China Center for Type Culture Collection, Wuhan University, Luojiashan Street, Wuchang District, Wuhan City, Hubei on July 2, 2007, wherein the preserving number is CCTCC NO: M207093. The invention also discloses a preparation method and application thereof. A Bacillus licheniformis 202 strain is obtained by screening through plate confronting and pot experiment, can effectively inhibit the growth of banana vascular wilt pathogens, has a wide antimicrobial spectrum, has antagonistic action on a plurality of pathogenic fungi such as cotton wilt pathogens, cotton verticillium wilt pathogens, watermelon wilt pathogens, tomato wilt pathogens, curvularia leaf spot pathogens or banana anthracnose pathogens, and has good environmental safety and good development and application prospect.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to an endogenous biocontrol bacterial strain, in particular to a bacillus licheniformis 202 for efficiently preventing and controlling banana wilt and its application. Background technique [0002] Banana Fusarium wilt is a devastating disease caused by the infection of Fusarium oxysporum f.sp.cubense, and it is a devastating soil-borne disease of banana production. The pathogen invades from the injured rhizome, spreads to the pseudostem and leaves through the vascular bundle, and blocks the xylem vessel until the plant withers and dies. Fusarium wilt of banana is a soil-inhabiting fungus, and its chlamydospores can survive in the soil for 8 to 10 years. Banana Fusarium wilt is distributed in many counties and cities in the banana production area of ​​the Pearl River Delta, and it has developed rapidly in recent years. The rate of diseased plants in the diseased garden is generally...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/10
Inventor 王振中孙正祥纪春艳
Owner SOUTH CHINA AGRI UNIV
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