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Coronary heart disease and palsy risk diagnosis reagent kit and manufacturing method thereof

A diagnostic kit and a technology for making a method, which are applied in the field of medicine and biology

Inactive Publication Date: 2009-05-27
SHANDONG TAIBANG BIOLOGICAL PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there are interventional techniques, stem cell technology, and drug-eluting stents for the treatment of coronary heart disease, experts emphasize that prevention is better than cure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The required main reagents and auxiliary supplies of the diagnostic kit of the present invention are as follows:

[0052] 1.1 Reagents

[0053] 1.1.1 Coating buffer: 0.05M (pH9.6) carbonate buffer

[0054] 1.1.2 Washing buffer: 0.15M (pH7.4) PBS

[0055] 1.1.3 Analysis buffer: (NH 4 ) 2 SO 4 , Tween-20 BSA

[0056] 1.1.4 Stop solution: 2M H 2 SO 4

[0057] 1.1.5 Substrate buffer: pH5.0 phosphate buffer

[0058] 1.1.6 TMB reagent: (3,3’,5,5’-tetramethylbenzidine), slightly acidic buffer

[0059] 1.1.7 Protein standard: the concentration is 1000ng / ml, 500ng / ml, 250ng / ml, 50ng / ml, 0ng / ml

[0060] 1.1.8 Capture antibody and enzyme-labeled detection antibody

[0061] 1.1.9 Normal human serum and positive control serum

[0062] 1.2 Supplements:

[0063] 1.2.1 96-well ELISA plate

[0064] 1.2.2 ELISA detector (450nm)

[0065] 1.2.3 96-well plate orbital shaker

[0066] 1.2.4 Microsampler

[0067] 1.2.5 Disposable sterile tips

[0068] 1.2.6 Small beakers, gla...

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PUM

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Abstract

The invention relates to the field of medical biotechnology, in particular to a making method for coronary heart disease and apoplexy risk diagnosis kit. The kit is used for quantitative analysis of Lp-PLA2 level in human plasma or blood serum by enzyme linked immunosorbent assay, so as to prevent heart disease and apoplexy.

Description

technical field [0001] The invention relates to the field of medical biotechnology, in particular to a method for making a coronary heart disease and stroke risk diagnostic kit. Background technique [0002] Statistics from the Ministry of Health: According to the results of disease surveillance in 36 cities across the country or some urban areas, chronic diseases are the main cause of death in the Chinese population, accounting for about 70% of the total number of deaths. Among them, cardiovascular and cerebrovascular diseases are the first, and nausea and tumors are the second. Second, diabetes has become the "third killer" that endangers human health. [0003] Cardiovascular and cerebrovascular diseases, as the "first killer" that endangers human health, have spread to the whole world and are the main disaster in many rich countries. Due to the difficulty in diagnosing certain types of coronary heart disease, the morbidity and mortality of acute myocardial infarction are...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/543
Inventor 林东柴希运庞广礼刘欣晏仲立军邵玉娟李西河李庆英
Owner SHANDONG TAIBANG BIOLOGICAL PROD CO LTD
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