Rapid propagation technique of Paris polyphylla var. yunnanensis, seedling exercising and planting methods
A kind of Dianzhonglou and technology technology, applied in the field of planting, Dianzhonglou rapid propagation technology and seedling hardening, can solve the problems such as no success and good effect, no breakthrough in planting survival rate, immature seedling hardening technology, etc. Achieve the effect of shortening the planting cycle, low production cost and ensuring consistency
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Embodiment 1
[0033] Example 1: Callus Induction and Differentiation
[0034] Take the robust exogenous body of P. edulis with high chemical composition of steroidal saponins, disinfect the surface with 70% ethanol for 1-6 minutes, then disinfect with 0.1% mercuric chloride for 5-15 minutes, wash with sterilized distilled water 6 times, 10 minutes each time. Then, the explants after the sterilization are cut into small pieces and inoculated on 0.2mg / L NAA and 1.5mg / L 6-BA hormone, mitogen 3% gac, add on the MS sucrose agar medium of 100ml / L coconut milk, The preparation process is according to the conventional method, the culture bottle is a glass bottle, and the sterilization adopts high-pressure moist heat sterilization at 121° C. for 30 minutes.
[0035] Placed in a light incubator, cultivated under the conditions of a temperature of 25°C±2, a light time of 12 hours, a light intensity of 1000-2000 lux, and μM m-2 s-1; The wounded tissue can be cultured for 4 weeks, and its volume can b...
Embodiment 2
[0037] Embodiment 2: induction of adventitious buds and adventitious roots
[0038] The calli were selected and transferred to the adventitious bud differentiation and subculture medium, placed in the conditions of temperature at 10°C±2, light time of 4 hours, light intensity of 1000-2000 lux, μM m-2 s-1 Adventitious buds were obtained under cultivation. According to this method, 45 days is a cycle of subculture.
[0039] Select rootless adventitious buds that grow robustly and have a height of 1.5-3.0 cm, transfer them to rooting medium, place them at a temperature of 20 ° C ± 2, light time for 10 hours, and light intensity of 2000-4000 lux, μM m Adventitious rooted seedlings were cultivated under the condition of -2 s-1, and the roots reached 3-5 cm.
Embodiment 3
[0040] Embodiment 3: seedling hardening
[0041]Trace element formula is adopted: the obtained rooting shoots are taken out from the rooting medium, and the medium is washed away with sterile water. Put the rooted seedlings in the trace element solution, add 4.2 grams of selenium, 5.6 grams of ammonium, 0.01 grams of zinc, and 0.5 grams of phosphorus per liter, and cultivate them for 15 days, keeping the light intensity at 4000-6000 lux, μM m-2 s-1 , the air temperature is 20-25°C, and the relative humidity is 70-100%. After 3-5 new lignified roots grow, transplant it to a greenhouse for 30-50 days to harden the seedlings, and 2-5 new fibrous roots can grow, and the length of the fibrous roots exceeds 0.5cm.
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