Rapid propagation technique of Paris polyphylla var. yunnanensis, seedling exercising and planting methods

A kind of Dianzhonglou and technology technology, applied in the field of planting, Dianzhonglou rapid propagation technology and seedling hardening, can solve the problems such as no success and good effect, no breakthrough in planting survival rate, immature seedling hardening technology, etc. Achieve the effect of shortening the planting cycle, low production cost and ensuring consistency

Inactive Publication Date: 2009-06-24
KUNMING YOULIFENGYUAN TECH DEV
View PDF0 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of seedlings by tissue culture can shorten the emergence cycle and make the species single, but the bottleneck problem that the tissue culture seedlings are not easy to take root, and the planting is not easy to survive has not been solved. There is no breakthrough in the survival rate of the tissue culture seedlings of D. , but there are no successful and good results report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Callus Induction and Differentiation

[0034] Take the robust exogenous body of P. edulis with high chemical composition of steroidal saponins, disinfect the surface with 70% ethanol for 1-6 minutes, then disinfect with 0.1% mercuric chloride for 5-15 minutes, wash with sterilized distilled water 6 times, 10 minutes each time. Then, the explants after the sterilization are cut into small pieces and inoculated on 0.2mg / L NAA and 1.5mg / L 6-BA hormone, mitogen 3% gac, add on the MS sucrose agar medium of 100ml / L coconut milk, The preparation process is according to the conventional method, the culture bottle is a glass bottle, and the sterilization adopts high-pressure moist heat sterilization at 121° C. for 30 minutes.

[0035] Placed in a light incubator, cultivated under the conditions of a temperature of 25°C±2, a light time of 12 hours, a light intensity of 1000-2000 lux, and μM m-2 s-1; The wounded tissue can be cultured for 4 weeks, and its volume can b...

Embodiment 2

[0037] Embodiment 2: induction of adventitious buds and adventitious roots

[0038] The calli were selected and transferred to the adventitious bud differentiation and subculture medium, placed in the conditions of temperature at 10°C±2, light time of 4 hours, light intensity of 1000-2000 lux, μM m-2 s-1 Adventitious buds were obtained under cultivation. According to this method, 45 days is a cycle of subculture.

[0039] Select rootless adventitious buds that grow robustly and have a height of 1.5-3.0 cm, transfer them to rooting medium, place them at a temperature of 20 ° C ± 2, light time for 10 hours, and light intensity of 2000-4000 lux, μM m Adventitious rooted seedlings were cultivated under the condition of -2 s-1, and the roots reached 3-5 cm.

Embodiment 3

[0040] Embodiment 3: seedling hardening

[0041]Trace element formula is adopted: the obtained rooting shoots are taken out from the rooting medium, and the medium is washed away with sterile water. Put the rooted seedlings in the trace element solution, add 4.2 grams of selenium, 5.6 grams of ammonium, 0.01 grams of zinc, and 0.5 grams of phosphorus per liter, and cultivate them for 15 days, keeping the light intensity at 4000-6000 lux, μM m-2 s-1 , the air temperature is 20-25°C, and the relative humidity is 70-100%. After 3-5 new lignified roots grow, transplant it to a greenhouse for 30-50 days to harden the seedlings, and 2-5 new fibrous roots can grow, and the length of the fibrous roots exceeds 0.5cm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method of Paris genus rapid propagation technique and seedling exercising, planting, comprising filtering a high grade individual system, sterilizing and tissue culture, wherein seedling exercising is the key to increase success ratio of the plant. The invention first performs Paris genus seedling exercising using microelement, causing the seedling exercising success ratio to reach 80%, thereby ensuring planting success ratio to reach 80%. The seedling exercising comprises: putting regeneration young seedlings in a culture medium added with vitamine and phytohormone, then adding microelement for cultivation and transplantation. The planting method comprises: transplanting the young seedlings through seedling exercising in sterilized prescription coil culture bag, when the newly put forth terminal bud develops into the blade, namely transplanted to the outside for insect control. The inventive technology and method ensure coherence of the germchit quality, ensuring high quality seed source of the Paris genus. The success ratio of the seedling exercising reaches 80%. The main root is thick and fibrous roots are increase, adaptability is strong in the field, the success ratio of the field reaches more than 80%, which solves problems of uneasy radication, immature seedling exercising technique, uneasy survival of planting. The invention is characterized by short vegetative cycle, low in production cost, good in quality and has scale production.

Description

technical field [0001] The present invention relates to a rapid propagation technique of Prunus diangi and methods for seedling hardening and planting. Background technique [0002] Chonglou is a species included in the "Chinese Pharmacopoeia". It is an important and scarce Chinese herbal medicine in the compatibility of Yunnan Baiyao and traditional Chinese medicine. , the resources are becoming increasingly exhausted. Although there are plantations in China, they germinate 1-2 years after sowing, and the provenance has not yet been resolved. With the rapid growth of the demand for raw materials of chrysanthemum by Chinese pharmaceutical companies, chrysanthemum has gradually become a rare species that is on the verge of extinction. endangered species. [0003] At present, there is no research report on this related technology in foreign countries. The research on the introduction and domestication of Chonglou and artificial cultivation technology in my country has begun s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00C12N5/04
Inventor 陈昌祥张艳张抱琼熊云昆石勇杨喆
Owner KUNMING YOULIFENGYUAN TECH DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap