Visible component counting pool, its production method and use method

A technology for counting pools and components, which is applied in the field of counting pools for formed components and detecting the density of formed components. It can solve the problems of manual multiple scrubbing, high price of professional counting pools, and threats to the health of operators, so as to reduce potential threats, simplify production, and Ease of use

Active Publication Date: 2009-07-08
BRED LIFE SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the price of commercialized professional counting pools is relatively high, especially for special-purpose counting pools such as sperm counting pools
Generally speaking, these commercialized professional counting pools are used repeatedly, requiring manual scrubbing of potentially infectious specimens in the counting pool, which will inevitably pose a threat to the health of the operators

Method used

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  • Visible component counting pool, its production method and use method
  • Visible component counting pool, its production method and use method
  • Visible component counting pool, its production method and use method

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Embodiment Construction

[0033] see Figure 1 ~ Figure 3 As shown, the formed component counting cell of the present invention is composed of a solid support plane 1 at the bottom, four support members 2 that control the counting depth in the middle—microspheres and a cover sheet 3 at the top, and the four microspheres are supported on the solid phase at the bottom. A square area for accommodating specimens to be detected is formed on the plane. The microspheres are adhered to the bottom solid-phase support plane with a high-viscosity, non-volatile vacuum silicone lipid material, and are not easy to fall off. The four microspheres in each counting cell have the same diameter, so the height of the supporting parts is the same, forming a uniform depth of the counting cell. However, for different counting pools, if the required counting depths are different, the needs can be achieved by selecting microspheres with different diameters.

[0034] The support member can be made of glass or metal, or plasti...

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Abstract

The invention relates to a counting cell for physical components, which comprises a bottom solid phase supporting plane (1), and three or more than three supporting components (2) for controlling counting depth are arranged on the bottom solid phase supporting plane. A manufacturing method thereof comprises the following steps: cleaning the supporting components; drying the supporting components; fully and evenly mixing high vacuum silicone ester and the supporting components according to a volume ratio of 2-15:1; and arranging the supporting components on the bottom solid phase supporting plane to form at least three supporting points. A using method comprises the following steps: adding samples (4) to be tested among the supporting components on the bottom solid phase supporting plane of the counting cell; covering the samples by a cover plate (3), and pressing the cover plate to the lowest position; and placing the counting cell below the microscope to count, wherein a computing formula comprises that: the density of the physical components is equal to average physical component number of each visual field divided by a product of the height of the supporting components multiplying the visual field area of the microscope. The counting cell has the advantages of low cost, simple manufacture, convenient use and long valid period, and can reduce threat of infectious samples to health by once use.

Description

technical field [0001] The present invention relates to a formed component counting device, in particular to a formed component counting pool and its manufacturing method and a method for using the counting pool to detect the formed component density. Background technique [0002] When the laboratory measures the density of cells, sperm or other small formed elements, it usually needs to use a professional counting pool (plate). Due to the difference in the distribution of the formed component density in different types of specimens, in order to ensure the reliability of the test results, different types of specimens need to use corresponding specialized counting pools, such as the detection of red and white blood cell density in blood samples. Blood cell counting pools, determination of sperm density in semen samples require the use of sperm counting pools, etc. However, not all types of specimens can obtain commercialized counting pools that match their determination need...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N33/48
Inventor 刘瑜
Owner BRED LIFE SCI TECH
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