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Method for producing blattella germanica allergen BgGSTD1 protein in bacilliform virus-insect expression system

A German cockroach and insect expression technology, applied in the field of genetic engineering, can solve the problem of no baculovirus-insect system expression, and achieve the effect of solving the complex purification process

Inactive Publication Date: 2011-01-05
JIANGSU PROVINCE HOSPITAL
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Problems solved by technology

At present, the allergen is only expressed in E. coli, but there is no report of expression in the baculovirus-insect system

Method used

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  • Method for producing blattella germanica allergen BgGSTD1 protein in bacilliform virus-insect expression system
  • Method for producing blattella germanica allergen BgGSTD1 protein in bacilliform virus-insect expression system
  • Method for producing blattella germanica allergen BgGSTD1 protein in bacilliform virus-insect expression system

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Abstract

The invention discloses a method for producing German cockroach allergen BgGSTD1 protein in rhabdovirus-insect expression system, comprising the following steps: total RNA is extracted from the tissue of the German cockroach, the total RNA is reversely transcripted into cDNA, a coding gene of German cockroach allergen BgGSTD1 is obtained by the method of PCR; the coding gene is cloned to a rhabdovirus carrier, insect cells are transformed for expression; the method of affinity chromatography is adopted for purification, thus obtaining the German cockroach allergen BgGSTD1 protein. In the invention, the rhabdovirus-insect expression line is adopted to produce the German cockroach allergen BgGSTD1 protein, thus avoiding influence on Bla g 7 allergen structures in different expression systems (such as bacterial and microzyme); in addition, the invention provides the method of affinity chromatography, thus solving the problem that the BgGSTD1 allergen has complex purification process.

Description

A method for producing German cockroach allergen BgGSTD1 protein in baculovirus-insect expression system technical field The invention belongs to the technical field of genetic engineering, and in particular relates to a method for producing the German cockroach allergen BgGSTD1 protein in a baculovirus-insect expression system. Background technique Allergic diseases are one of the major human diseases. Its incidence rate is currently estimated to account for 30-40% of the world's population, and is increasing at a rate of more than 1% per year, with the most obvious increase in the incidence rate of children. Over the past few decades, the incidence of allergic diseases has increased significantly and rapidly globally, more in Western than in developing countries, and more in urban than in rural areas. Taking allergic asthma (asthma) as an example, there are currently 5.2 million asthmatic patients in the UK: 1.1 million children (1 / 10 of the total population of children...

Claims

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Application Information

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IPC IPC(8): C12N15/866C12R1/93
Inventor 魏继福何韶衡
Owner JIANGSU PROVINCE HOSPITAL
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