Morphine quantitative and micro detection method

The invention relates to a method for quantitatively detecting morphine concentration with surface enhancement raman spectrum (SERS for short), and belongs to the technical filed of medicine and narcotic drug detection. The method comprises the following steps: preparation of an active SERS substrate, dissolving AgNO3 in water to prepare AgNO3 solution, adding trisodium citrate after heating to 78-92 DEG C, and stirring for 60min to obtain green yellow silver colloid; centrifuging the silver colloid at a high speed to obtain high concentration silver colloid; and mixing aqueous morphine solution with part of the high concentration silver colloid for raman spectrum test to obtain a morphine-silver colloid SERS spectrogram; performing raman spectrum test on the rest high concentration silver colloid background to obtain a silver colloid background SERS spectrogram; correcting baseline of the spectrum peak of the two spectrograms by a computer to obtain a high quality morphine SERS spectrogram; contrasting with a standard morphine raman spectrum strength-concentration curve to obtain the concentration of the morphine. The method has the advantages of short measurement and analysis time, small amount of sample and convenient and accurate detection.