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Morphine quantitative and micro detection method

A detection method, morphine technology, applied in the field of quantitative and trace detection of morphine, to achieve the effect of less sample consumption, convenient detection and short time

Inactive Publication Date: 2009-07-29
FUJIAN NORMAL UNIV
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Problems solved by technology

[0003] The Raman spectrum detection of morphine has not been reported, especially the detection of morphine with surface-enhanced Raman spectroscopy, which is still blank in China

Method used

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  • Morphine quantitative and micro detection method
  • Morphine quantitative and micro detection method
  • Morphine quantitative and micro detection method

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Embodiment 1

[0019] 1. Take 90mg of AgNO 3 Dissolved in 500mL of water to prepare AgNO with a mass percentage of 0.018% 3 Aqueous solution, when the aqueous solution was heated to 90°C, 10mL of trisodium citrate aqueous solution with a mass percentage of 1% was added dropwise, and stirred for 60min at the same time to obtain a green-yellow silver colloid.

[0020] 2. Put an appropriate amount of silver colloid into a centrifuge tube and centrifuge at high speed for 10 minutes, and remove the layer of high-concentration silver colloid for later use.

[0021] 3. Weigh 0.9mg of morphine, add it into a 1000mL volumetric flask, dilute to 1000mL with ultrapure water, and prepare a 900ng / mL morphine aqueous solution. Measure 5mL of the above-mentioned morphine solution and mix it with 5mL of high-concentration silver colloid. image 3 The morphine-silver colloidal SERS spectrum shown. After the morphine solution is mixed with the silver colloid, the obvious colloidal aggregation phenomenon in ...

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Abstract

The invention relates to a method for quantitatively detecting morphine concentration with surface enhancement raman spectrum (SERS for short), and belongs to the technical filed of medicine and narcotic drug detection. The method comprises the following steps: preparation of an active SERS substrate, dissolving AgNO3 in water to prepare AgNO3 solution, adding trisodium citrate after heating to 78-92 DEG C, and stirring for 60min to obtain green yellow silver colloid; centrifuging the silver colloid at a high speed to obtain high concentration silver colloid; and mixing aqueous morphine solution with part of the high concentration silver colloid for raman spectrum test to obtain a morphine-silver colloid SERS spectrogram; performing raman spectrum test on the rest high concentration silver colloid background to obtain a silver colloid background SERS spectrogram; correcting baseline of the spectrum peak of the two spectrograms by a computer to obtain a high quality morphine SERS spectrogram; contrasting with a standard morphine raman spectrum strength-concentration curve to obtain the concentration of the morphine. The method has the advantages of short measurement and analysis time, small amount of sample and convenient and accurate detection.

Description

Technical field [0001] The invention relates to quantitative and trace detection of morphine and a method thereof, in particular to a method for quantitatively detecting the concentration of morphine by using surface-enhanced Raman spectroscopy (SERS for short), belonging to the technical field of drug and drug detection. Background technique [0002] Since the discovery by Fleischmann et al., surface-enhanced Raman scattering has become one of the most sensitive detection techniques for molecular absorption and single-molecule detection. Many analytes at ultra-low concentrations, including chemical, biological, and medical molecules, have been detected by SERS. When target molecules are adsorbed on silver or gold nanostructures, the non-resonant Raman signal is enhanced by up to 10 14 times. Raman spectroscopy and SERS have been widely used in biomedical research. Morphine (C 17 h 19 NO 3 ) is a powerful analgesic but also a common drug that is attracting the interest...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 冯尚源黄伟陈荣李永增黄祖芳林居强俞允陈杰斯
Owner FUJIAN NORMAL UNIV
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