Method for analyzing fatty acid chemical composition in cortex periplocae radicis

A technology of chemical composition and fatty acid, which is applied in the field of analysis of fatty acid chemical composition in Persica sagepipes by GC-MS technology, and can solve the problems of extraction and analysis of unseen fatty acid components

Active Publication Date: 2009-08-05
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no reports about the extraction and analysis of its fatty acid components at home and abroad.

Method used

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  • Method for analyzing fatty acid chemical composition in cortex periplocae radicis
  • Method for analyzing fatty acid chemical composition in cortex periplocae radicis
  • Method for analyzing fatty acid chemical composition in cortex periplocae radicis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1 Apparatus, materials and reagents

[0020] 1.1 Instrument GC-MS combined instrument (SHIMADZU 6010N GC System / SHIMADZU6010N MS System); AGBP210S electronic balance (German satorious company).

[0021] 1.2 The medicinal materials and reagents Xiangjiapi, purchased from Shenyang Medicinal Materials Company, were identified as genuine by Professor Sun Qishi, Department of Chinese Medicine, Shenyang Pharmaceutical University. All reagents used are analytical reagents.

[0022] 2 Experimental part

[0023] 2.1 Fatty acid extraction 100g of Perilla bark medicinal materials, crushed and refluxed with petroleum ether (60~90℃) for 8h, filtered, concentrated under reduced pressure, and evaporated to dry solvent to obtain fatty acid.

[0024] 2.2 Sample methyl esterification Take 2 drops of fatty acid samples, add 2 mL of 2% sodium hydroxide-methanol solution and place in a 60℃ water bath for 15 minutes, then add 2 mL of boron trifluoride-ether solution to react for 5 minutes. Accur...

Embodiment 2

[0035] 1 Apparatus, materials and reagents

[0036]1.1 Instrument GC-MS combined instrument (SHIMADZU6010N GC System / SHIMADZU6010N MS System); AGBP210S electronic balance (German satorious company).

[0037] 1.2 Medicinal materials and reagents Fragrant cassia peel, purchased from Shenyang Medicinal Materials Company, was identified as a genuine product by Professor Sun Qishi, Department of Chinese Medicine, Shenyang Pharmaceutical University. All reagents used are analytical reagents.

[0038] 2 Experimental part

[0039] 2.1 Extraction of fatty acids: 100g of the herbal medicine of Perilla peel, pulverized and refluxed with petroleum ether (60℃~90℃) for 6h, filtered, concentrated under reduced pressure, and evaporated to dry the solvent to obtain fatty acids.

[0040] 2.2 Sample methyl esterification Take 2 drops of fatty acid samples, add 2 mL of 2% sodium hydroxide-methanol solution and place in a 60℃ water bath for 15 minutes, then add 2 mL of boron trifluoride-ether solution...

Embodiment 3

[0047] 1 Apparatus, materials and reagents

[0048] 1.1 Instrument GC-MS combined instrument (SHIMADZU 6010N GC System / SHIMADZU6010N MS System); AGBP210S electronic balance (German satorious company).

[0049] 1.2 Medicinal materials and reagents Fragrant cassia peel, purchased from Shenyang Medicinal Materials Company, was identified as a genuine product by Professor Sun Qishi, Department of Chinese Medicine, Shenyang Pharmaceutical University. All reagents used are analytical reagents.

[0050] 2 Experimental part

[0051] 2.1 Extraction of Fatty Acids 100g of Herba Perfumidae, pulverized and refluxed with petroleum ether (60℃~90℃) for extraction for 10 hours, filtered, concentrated under reduced pressure, and evaporated to dry the solvent to obtain fatty acids.

[0052] 2.2 Sample methyl esterification Take 2 drops of fatty acid samples, add 2 mL of 2% sodium hydroxide-methanol solution and place in a 60℃ water bath for 15 minutes, then add 2 mL of boron trifluoride-ether solut...

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Abstract

The invention provides a method for analyzing chemical components of fatty acid in cortex periplocae radicis. The method includes the following steps: (a) the fatty acid is extracted from the cortex periplocae radicis; (b) methyl ester is performed for the fatty acid; (c) the methyl esterifed fatty acid is detected by a GC-MS combination method, wherein the chromatographic condition for GC-MS combination is as follows: the chromatographic column is a quartz capillary chromatographic column; the carrier gas is a high-purity helium gas; the temperature of an introduction port is 230-270 DEG C; the split ratio is 8:1-12:1; and the flow speed is 0.8-1.2mL.min. The mass spectrum condition is as follows: the ion source is an EI ion source; the temperature of a tetrode is 120-170 DEG C; the temperature of a transmission line is 270-310 DEG C; the voltage of a multiplier tube is 1800-2200V; and the temperature of a GC-MS interface is 210-250 DEG C. Being tested by a methodology, the method is accurate and reliable and can be used for analyzing the chemical components of fatty acid in cortex peripolcae radicis.

Description

Technical field [0001] The invention relates to a method for analyzing the chemical constituents of fatty acids in the peels, in particular to a method for analyzing the chemical constituents of the fatty acids in the peels by using GC-MS combined technology. Background technique [0002] Periploca speium (Periploca speium), also known as Acanthopanax senticosus and Willow bark, is the dried root bark of Periploca sepium Bge, a plant of the family Asclepiaceae. Fragrant plus skin tastes pungent, bitter and warm; toxic, returning to the liver, kidneys, and heart meridians, can dispel rheumatism, strengthen muscles and bones, and be used to treat rheumatic arthralgia, waist and knee pain, palpitation, shortness of breath, and lower limb edema. Modern clinical use for the treatment of rheumatoid arthritis, breast cancer, etc.; with its total saponins for the treatment of chronic congestive heart failure (Deng Shixian, Wang Decheng, Wang Maode, etc.. Dianluoside cardiac effect[J].Act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/02
Inventor 李莉佟玲周水平高钧马洁毕开顺
Owner TIANJIN TASLY PHARMA CO LTD
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