Method for producing fungal granular formulation for preventing and treating pest

A technology of granules and granules, applied in the fields of botanical equipment and methods, biocides, biocides, etc., can solve the problem of infestation of target bacteria or weeds affecting the growth of beneficial fungi, difficult to accurately calculate the bacterial content of granules, Environmental microbial contamination and other problems, to achieve the effect of maintaining the vitality of fungi, conducive to infection control, and maintaining biological activity

Inactive Publication Date: 2011-07-27
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing research and existing problems: According to the characteristics of plant growth and environment and the requirements of fungal application operations, entomopathogenic fungi are generally made into powders, granules, and liquids, which can be used for spreading, spraying, spraying, etc.
[0008] The advantage of the granule prepared by the prior art is that it can keep the granule shape for a long time, and the fungus in the granule can resume growth under suitable conditions simultaneously, but there are still the following deficiencies: 1) the fungal culture used is mycelium, which is liquid It is directly obtained by fermentation, and the biomass per unit volume usually has a large change. The method provided is to mix it with sodium alginate in a certain volume ratio, so it is difficult to accurately calculate the actual bacterial content of the granules prepared each time; 2) the used The fungal culture is mycelium, which is relatively easy to inactivate during the drying and storage of granules; 3) Since no carrier is added, the mixture of bacteria and sodium alginate is directly added dropwise to CaCl 2 In the aqueous solution, the granules formed are usually no longer spherical after drying, but are flat cakes or flakes, which is unfavorable for throwing and spreading; The number of colonies grown is not high, that is, the number of colonies grown per unit of bacteria is low, and the amount and cost of controlling diseases or weeds in the field are relatively high; 5) Sodium alginate can be decomposed and utilized by microorganisms in the environment, according to The granules prepared by the prior art method are easily and quickly polluted by environmental microorganisms after being stored and sprinkled in the field, directly affecting the growth of beneficial fungi in the granules and their infecting effect on target bacteria or weeds

Method used

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  • Method for producing fungal granular formulation for preventing and treating pest
  • Method for producing fungal granular formulation for preventing and treating pest
  • Method for producing fungal granular formulation for preventing and treating pest

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Cultivate Metarhizium anisopliae from Potato Dextrose Medium Agar to obtain dry and uniform Metarhizium anisopliae conidia powder.

[0030] 2) Take 5 g of spore powder, 85 g of diatomaceous earth, and 10 g of corn flour respectively, and premix them uniformly to form mixture I;

[0031] 3) Add mixture I to 400mL 2% (w:v) sodium alginate aqueous solution, mix well to form mixture II;

[0032]4) Add the mixture II dropwise into 0.25M calcium chloride aqueous solution with a syringe, let it stand for 15 minutes for curing reaction, and shake slowly several times occasionally;

[0033] 5) Remove the particles and transfer them to an aqueous solution containing 2% (w: v) sodium alginate and 6% (w: v) polyvinyl alcohol, and then transfer them to a solution containing 0.25M calcium chloride and saturated boric acid after a few minutes. in the mixed aqueous solution, let stand for 5 minutes;

[0034] 6) Remove the granules, rinse them with sterilized water once, then rinse...

Embodiment 2

[0036] 1) Cultivating Beauveria bassiana on the wheat bran straw powder medium, and obtaining dry and uniform Beauveria bassiana conidia powder through separation and removal of impurities;

[0037] 2) Weigh 10 g of spore powder, 88 g of attapulgite, and 2 g of rice flour, and pre-mix evenly to form mixture I;

[0038] 3) Add mixture I to 400mL of 1% (w:v) sodium alginate aqueous solution, and mix well to form mixture II;

[0039] 4) Add the mixture II dropwise into 0.28M calcium chloride aqueous solution with a funnel with a controlled dropper, let it stand for 20 minutes for the curing reaction, and shake it gently several times occasionally;

[0040] 5) Remove the particles and transfer them to an aqueous solution containing 1% (w: v) sodium alginate and 5% (w: v) polyvinyl alcohol, and then transfer them to a solution containing 0.28M calcium chloride and saturated boric acid after a few minutes. in the mixed aqueous solution, let stand for 5 minutes;

[0041] 6) Remove ...

Embodiment 3

[0043] 1) Cultivate Verticillium lecanii on the rice grain medium, and obtain dry and uniform conidia powder of Verticillium lecanii through separation and removal of impurities;

[0044] 2) Weigh 10 g of spore powder, 80 g of attapulgite, and 10 g of soluble starch, and premix evenly to form mixture I;

[0045] 3) Add mixture I to 250mL of 1% (w:v) sodium alginate aqueous solution, mix well to form mixture II;

[0046] 4) Add the mixture II dropwise to 0.25M calcium chloride aqueous solution with a pressurizable dropper, let it stand for 30 minutes for curing reaction, and shake it gently several times occasionally;

[0047] 5) Remove the particles and transfer them to an aqueous solution containing 1.5% (w: v) sodium alginate and 3.5% (w: v) polyvinyl alcohol, and then transfer them to a solution containing 0.25M calcium chloride and saturated boric acid after a few minutes. in the mixed aqueous solution, let stand for 10 minutes;

[0048] 6) Remove the granules, rinse the...

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Abstract

The present invention relates to a method for manufacturing a fungal granule for controlling insects. The invention provides a method for preparing an entomopathogenic fungi sodium alginate granule and a granular formulation prepared through the method. Compared with the prior art, the invention not only preserves the normal advantages of long-time shape keeping of algae colloidal particle and growth recovering of fungus in the particles, but also has other innovations and improvements on other aspects. The algae colloidal particles containing fungus are used for being sprinkled to the farmland or woodland for controlling insects.

Description

technical field [0001] The invention relates to a method for producing a microbial preparation and the microbial preparation prepared by the method. Background technique [0002] The use of entomopathogenic fungi to control agricultural and forestry pests has been widely studied and discussed. So far, a variety of entomopathogenic fungi including Beauveria bassiana coccidioides, Metarhizium anisopliae, Verticillium lecanii, Mortierella Thomsonii, etc. have been used as biopesticides for the control of dozens of pests, including Lepidoptera , Coleoptera, Orthoptera, Homoptera and other subjects of insects and mites. Some have been developed into biopesticide preparations, which have been registered as pesticides in different countries and recommended by FAO, playing an active role in protecting plants, reducing the use of chemical pesticides, and maintaining ecological balance. [0003] It is the key to making entomopathogenic fungi into certain forms of preparations to eff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N25/26A01N25/08A01P7/00
Inventor 农向群张泽华高松王广君
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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