Processing procedure for peripheral blood stem cells

A stem cell, peripheral blood technology, applied in blood/immune system cells, animal cells, vertebrate cells, etc.

Inactive Publication Date: 2009-08-12
NEOSTEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the application of these techniques is limited because the total number of stem cells in the peripheral

Method used

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  • Processing procedure for peripheral blood stem cells
  • Processing procedure for peripheral blood stem cells
  • Processing procedure for peripheral blood stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0067] refer to figure 1 , step 10 of the present invention in combination with an embodiment of the present invention generally includes the following steps:

[0068] (1) Healthy stem cells are collected while the individual is in the "pre-disease" stage (12). By "pre-disease" is meant that the individual is healthy, or that the individual develops, manifests, or is diagnosed with any or a specific disease that is known to affect the quality of stem cells, or that the individual Said individual is considered to be in a certain health condition, so that it cannot collect stem cells of a certain quality.

[0069] (2) The cells in the pre-disease state are preserved and stored in a stem cell bank (14), eg, by freezing, for many years, eg, 500 years or less. Preferably, the cells in the pre-disease state are preserved and maintained in a stem cell bank throughout the life of the individual, after which time the cells may be discarded or used for allogeneic transplantation or ot...

Embodiment approach

[0161] Therefore, an embodiment provided by this invention: when the non-neonatal or adult is in the pre-disease stage, the stem cells of the non-neonatal or adult are collected and stored (for example: cryopreservation), and the method of collecting stem cells can be used Plasmapheresis is achieved. Injection of growth factors, such as G-CSF, is required 1-6 days before harvesting the stem cells. According to a preferred embodiment: non-newborns or adults are given cell growth factors for two consecutive days, and peripheral blood stem cells are separated by plasmapheresis on the third day. In order to store the collected stem cells for future use, freezing techniques and cryogens can be applied.

[0162] Later (for example, years later) once the person with preserved stem cells develops cancer, immune disease, infectious disease, heart disease, helix disease, pancreas disease, liver disease, or bone marrow disease or encounters a condition that causes immunosuppression due ...

Embodiment 1

[0242] Example 1: Collection and cryopreservation of peripheral blood stem cells

[0243] A patient whose stem cells are to be preserved for future autologous transfer is selected, and peripheral blood stem cells from that patient are collected. Briefly, the patient's vital medical parameters were collected, including age (45 years), weight (150 lbs), sex (male), blood pressure (120 / 80) and hematocrit value (42%). The total blood volume of the donor was calculated from the sex, height and weight of the donor, and the process volume of total blood per cycle was determined from the hematocrit value. The centrifugal speed of the blood separator was set at 4800rpm, and the critical flow rate was set at 50ml / min.

[0244] The patient's stem cells were collected for 3 hours. The total volume of cells collected was 250ml. These cells were washed with sterile RPMI medium and resuspended in cryopreservation medium (RPMI+10% glycerol+5% DMSO). Half of the cells were aliquoted into 2...

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Abstract

An elective healthcare insurance model using an individual's own peripheral blood stem cells for the individual's future healthcare uses. An individual can elect to have his or her own stem cells collected, processed and preserved, while he or she is in healthy or ''pre-disease'' state, for future distribution for his or her healthcare needs. The process includes methods of collection, processing, preservation and distribution of adult (including pediatric) peripheral blood stem cells during non-diseased state. The stem cells collected will contain adequate dosage amounts, for one or more transplantations immediately when needed by the individual for future healthcare treatments. The collected adult or non-neonate child peripheral blood stem cells can be aliquoted into defined dosage fractions before cryopreservation so that cells can be withdrawn from storage without the necessity of thawing all of the collected cells.

Description

[0001] related application [0002] This invention claims U.S. Provisional Application 60 / 814,235 filed June 15, 2006, filing date, U.S. Provisional Application 60 / 815,399 filed June 20, 2006, U.S. Provisional Application 60 / 839,311 filed August 21, 2006 priority of these applications, the entire contents of which are hereby incorporated by reference. technical field [0003] The present application relates to methods of making and using autologous adult stem cells from non-disease or pre-disease subjects. Background technique [0004] Stem cell transplantation has been used alone (eg for congenital diseases) or in combination with other treatments (eg chemotherapy). The widespread use of stem cells is due to their ability to regenerate one or more cell lineages when administered to a patient. Most stem cell transplants use stem cells provided by a matched donor (allogeneic) or stem cells from the patient (autologous) collected immediately before the patient undergoes trea...

Claims

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Application Information

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IPC IPC(8): C12N5/06A61K35/14A61K35/28C12N5/0789
Inventor D·O·罗杰森G·S·史密斯R·E·阿林W·A·马拉斯科
Owner NEOSTEM
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