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In-vitro culture method of antlerpilose grass

A technology of in vitro culture of velvet velvet, applied in the field of in vitro culture of velvet velvet, can solve the problem of small quantity of velvet velvet, achieve the effect of rapid supply, increase planting yield, and accelerate the promotion of improved varieties

Inactive Publication Date: 2009-08-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Scrophulariaceae belongs to the genus Scrophulariaceae. It has the functions of clearing heat and detoxifying, expelling wind and promoting qi, cooling blood and stopping bleeding, and eliminating phlegm. It is used to treat colds, chronic bronchitis, pneumonia, etc., and has high medicinal value; it is distributed in In the areas of Jiangsu and Zhejiang, artificial cultivation has not yet been realized in China, so the number of wild pilose antler grass is getting less and less

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, a kind of method for in vitro culture of pilose antler grass, carries out following steps successively:

[0022] 1) Put the young shoots of velvet antler grass without disease spots and grow vigorously into a beaker, and rinse with tap water for 1 to 2 hours;

[0023] 2), the above-mentioned shoots are routinely sterilized (i.e. 0.1% w / v HgCl 2 After processing for 6~10 minutes, wash 5~8 times with sterile water, then blot dry with sterile filter paper), cut into small sections of buds 1 about 1cm, inoculate on the induced adventitious bud medium and cultivate; the culture condition is : 16 hours of light, light intensity 10 ~ 30μmol m -2 .s -1 , the temperature is 26-28°C; 8 hours of dark cultivation, the temperature is 20-22°C; the above-mentioned light and dark cultivation are carried out alternately;

[0024] The medium for inducing adventitious buds is: MS basic medium + BA 0.5-1.0 mg / l + sugar 20-30 g / l + agar 5-9 g / l, and the pH is 5.5-6.0.

[0...

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PUM

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Abstract

The invention discloses an isolated culture method for Monochasma Savatieri Franch.ex Maxim, which comprises the following steps in turn: 1) washing a burgeon of the Monochasma Savatieri Franch.ex Maxim; 2) after conventional disinfection, cutting the burgeon into a small segment of burgeon I and inoculating the small segment of burgeon I to an adventitious bud culture medium for culture; 3) cutting an adventitious bud growing out on the small segment of burgeon I, and inoculating the obtained small segment of burgeon II to a multiplication culture medium for culture; 4) cutting an adventitious bud growing out on the small segment of burgeon II, and inoculating an obtained small segment of burgeon III to a multiplication culture medium for culture; 5) cutting an adventitious bud growing out on the small segment of burgeon III, and inoculating an obtained seedling III into a rooting culture medium; and 6) obtaining the seedling capable of being planted out of a bottle until the seedling III grows out at least three roots greater than 2 cm. With the method, people can obtain a great number of seedlings of the Monochasma Savatieri Franch.ex Maxim.

Description

technical field [0001] The invention relates to a method for in vitro cultivation of pilose antler grass. Background technique [0002] Scrophulariaceae belongs to the genus Scrophulariaceae. It has the functions of clearing heat and detoxifying, dispelling wind and promoting qi, cooling blood and stopping bleeding, and eliminating phlegm. It is used to treat colds, chronic bronchitis, pneumonia, etc., and has high medicinal value; it is distributed in In the areas of Jiangsu and Zhejiang, artificial cultivation has not yet been realized in China, so the number of wild pilose antler grass is decreasing. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for in vitro cultivation of velvet grass, by which a large number of seedlings of velvet grass can be obtained. [0004] In order to solve the above-mentioned technical problems, the present invention provides a method for in vitro culture of velvet velvet ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张国芳冯利毛碧增
Owner ZHEJIANG UNIV