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Method for freeze preservation of tissue-derived cell

A cryopreservation and tissue technology, which is applied to the preservation of human or animal bodies, tissue culture, animal cells, etc., can solve the problems of labor and time, and achieve the effect of low-cost and high-efficiency acquisition

Inactive Publication Date: 2009-09-02
CELL BANK
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Therefore, cultivating recovered cells using a selection medium grown only from specific cells takes effort and time for selecting target cells
In addition, in the case of the method of immersing the tissue fragment itself in the culture medium and waiting for the cells to migrate from the tissue to the culture medium, since a long period of culture is required and subsequent steps are required to separate the cells obtained after migration from the tissue, Generation, so it takes effort and time

Method used

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  • Method for freeze preservation of tissue-derived cell
  • Method for freeze preservation of tissue-derived cell
  • Method for freeze preservation of tissue-derived cell

Examples

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Embodiment 1

[0039] Three adult females (ages 33, 27, and 35 years old) and one adult male (age 50 years old) obtained their skins and performed the following experiments with their own consent. After each subject was anesthetized, a full-thickness skin of 1 mm to 3 mm was extracted from the back of the auricle. Immediately perform 2-hour enzyme (dispase2000 unit / ml, contract alcohol) treatment, separate the full-thickness skin into epidermis and dermis, discard the epidermis, and finely cut the dermis (about 0.5mm in diameter) to make 10 sliced ​​specimens. The obtained 10 specimen slices were divided into 2 groups for the following experiments.

[0040] Group D (direct group): The specimen sections were immersed in cryopreservation solution (10% DMSO, 80% αMEM, 10% human serum) or commercially available cell cryopreservation solution Cellbanker (registered trademark) 1 (Shici) without any treatment. Field Co., Ltd.), stored at -80°C for 12 hours, and then frozen in liquid nitrogen for 2...

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Abstract

An object of the present invention is to provide a method for cryopreservation of desired cells derived from various tissues simply and effectively. The method comprises: dividing a tissue containing cells of interest into pieces; culturing the divided tissue pieces in a predetermined culture medium; collecting the cultured tissue pieces; suspending the cultured tissue pieces in a cryoprotective solution; and freezing the resulting suspension of the tissue pieces at a temperature of -70 DEG C or lower. In a preferred mode, the cells are fibroblasts derived from skin tissue.

Description

technical field [0001] The present invention relates to a method for cryopreserving tissue-derived cells, and more particularly, to a method for conveniently and efficiently cryopreserving desired cells derived from various tissues obtained from mammals. [0002] This application claims the priority of Japanese Patent Application No. 2006-274243 (filing date: October 5, 2006), and incorporates all the descriptions of this application into this application document by reference. Background technique [0003] Mammalian tissues or organs are used to investigate the efficacy of transplanted tissues, pharmaceuticals, or cosmetics to fill in defective parts of patients after they are removed from the subject and left untreated, or cultured and multiplied. In addition, applications to regenerative medicine, in which stem cells are extracted from specific tissues and used for proliferation, are also attracting attention. In general, tissues obtained from living bodies or cultured t...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N1/04C12N5/07C12N5/071
CPCA01N1/0205C12N1/04A01N1/02A01N1/021A01N1/0284C12N5/0602
Inventor 北条元治
Owner CELL BANK
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