Method for freeze preservation of tissue-derived cell
A cryopreservation and tissue technology, which is applied to the preservation of human or animal bodies, tissue culture, animal cells, etc., can solve the problems of labor and time, and achieve the effect of low-cost and high-efficiency acquisition
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0038] Example 1
[0039] Three adult females (aged 33, 27, and 35 years old) and one adult male (aged 50 years old), with my consent, took the skin and performed the following experiments. After the subjects were anesthetized, a full-thickness skin of 1 mm to 3 mm was taken from the back of the auricle. Enzyme treatment (dispase 2000 units / ml, contract alcohol) was carried out immediately to separate the full-thickness skin into the epidermis and dermis. After discarding the epidermis, the dermis (diameter about 0.5 mm) was cut into pieces to make 10 specimens. The 10 specimen sections obtained were divided into 2 groups for the following experiments.
[0040] Group D (direct group): The specimen sections are immersed in cryopreservation solution (10% DMSO, 80% αMEM, 10% human serum) or the commercially available cell cryopreservation solution cellbanker (registered trademark) 1 (Shici) without any treatment. Field Co., Ltd.), after storage at -80°C for 12 hours, it was frozen wi...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap