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Method for assaying nucleic acids by fluorescence

一种荧光测定、核酸的技术，应用在荧光测定法领域，能够解决必需提取、高操作成本、灵敏性不足等问题

Inactive Publication Date: 2009-09-02
BIOQUANTA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is not without drawbacks: necessary extraction, relatively high operating costs, specific equipment not available for large-scale routine analysis, and insufficient sensitivity
However, the technical conditions required to use the method (microplates, large amount of reagents, measuring fluorescence) make it difficult to reach the desired sensitivity limit and do not allow identification of the origin (human or non-human origin) of the detected DNA

Method used

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  • Method for assaying nucleic acids by fluorescence
  • Method for assaying nucleic acids by fluorescence
  • Method for assaying nucleic acids by fluorescence

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Embodiment Construction

[0066] nucleic acid

[0067] Nucleic acids may be of natural or synthetic origin, and the nucleotides to which they are incorporated, especially ribonucleotides or deoxyribonucleotides, may be natural or modified. Preferably, the nucleic acid is DNA or RNA, more preferably DNA. Also preferably, the size of the nucleic acid strand is greater than five bases.

[0068] Advantageously, the specific quantification of nucleic acids in a sample with low molecular weight strands (e.g., less than 1,000 bases in length) (optionally after selecting these nucleic acids using a suitable method) allows quantification, for example, of the biological or cultural death due to apoptosis of cells in an organism; the value thus measured can be compared to the value of the total amount of DNA, optionally measured without the use of any method for selecting strands less than 1,000 base pairs in size correlated to provide the percentage of cell death due to apoptosis.

[0069] Apoptosis can also ...

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Abstract

The invention relates to a method for determining the amount of nucleic acid present in a sample, wherein: - a fluorophore is added to the sample, - fluorescence intensities emitted by the fluorophore at at least two emission wavelengths in response to light stimulations at at least two excitation wavelengths respectively are measured, and - the amount of nucleic acid present in the sample is deduced from the measured fluorescence intensities.

Description

[0001] This application claims the benefit of earlier US Provisional Application 60 / 836,949, filed August 11,2006. field of invention [0002] The present invention relates to a fluorometric method for determining the amount of nucleic acid present in a sample, to a fluorometer suitable for carrying out said method, and to a tray for the fluorometric method of compounds. Background of the invention [0003] The presence of cell-free nucleic acids in blood circulation has been known for several years [Mandel and Metais, 1947; Tan et al., 1966]. Since this time, cancer patients have been found to have high levels of circulating DNA relative to patients with benign tumors or relative to healthy subjects using methods such as RIA (radioimmunoassay) [Leon et al., 1977; Shapiro et al., 1983]. [0004] However, in recent years, with the development of efficient molecular biology methods such as real-time PCR, interest in considering circulating DNA fragments as clinical biomarkers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
CPCG01N2021/6419C12Q1/68C12Q1/6816G01N21/6428G01N2021/6421G01N2021/6482G01J3/4406C12Q2563/173C12Q2563/107C12Q2537/101C12Q2523/313
Inventor M·萨科N·德拉科特MS·巴赫M·孔蒂
Owner BIOQUANTA
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