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Immobilized porcine pancreatic lipase carrier, preparation method and application thereof

A technology of porcine pancreatic fat and enzyme carrier, applied in directions such as being fixed on/in an organic carrier, can solve the problems of complicated carrier preparation, harsh reaction conditions, difficult industrialization, etc. The effect of high annual output

Inactive Publication Date: 2009-09-16
SICHUAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Another object of the present invention is to provide a method for preparing the above-mentioned immobilized porcine pancreatic lipase carrier, which can overcome the disadvantages of complicated carrier preparation, harsh reaction conditions, high cost, and difficulty in industrialization in the prior art.

Method used

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  • Immobilized porcine pancreatic lipase carrier, preparation method and application thereof
  • Immobilized porcine pancreatic lipase carrier, preparation method and application thereof
  • Immobilized porcine pancreatic lipase carrier, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This embodiment is the immobilized porcine pancreatic lipase carrier prepared by the following method:

[0057] Surfactants Span (Span) 40 and Span80 are used as composite dispersants, kerosene is used as dispersed phase, acrylic acid (AA) is used as reactive monomer, and N,N'-methylenebisacrylamide (MBAA) is used as crosslinking agent , Ethylene glycol is the porogen, and the macroporous bead-shaped cross-linked polymer is prepared by reverse-phase suspension polymerization technology, and then activated by N-hydroxysuccinimide (NHS), to obtain the immobilized porcine pancreatic fat Enzyme carrier.

[0058] The raw materials used include:

[0059] Reactive monomer acrylic acid (AA) 5mL (about 5.3g),

[0060] Cross-linking agent N, N'-methylenebisacrylamide (MBAA) 2.1g (about 40% of AA quality),

[0061] Porogen ethylene glycol 4.0mL (about 4.5g, about 85% of AA mass),

[0062] The consumption ratio of dispersant Span40 and Span80 is adjusted to 5 with the HLB value...

Embodiment 2

[0085] This embodiment is the immobilized porcine pancreatic lipase carrier prepared by the following method:

[0086] Surfactants Span (Span) 40 and Span80 are used as composite dispersants, kerosene is used as dispersed phase, acrylic acid (AA) is used as reactive monomer, and N,N'-methylenebisacrylamide (MBAA) is used as crosslinking agent , Ethylene glycol is the porogen, and the macroporous bead-shaped cross-linked polymer is prepared by reverse-phase suspension polymerization technology, and then activated by N-hydroxysuccinimide (NHS), to obtain the immobilized porcine pancreatic fat Enzyme carrier.

[0087] The raw materials used include:

[0088] Reactive monomer acrylic acid (AA) 10mL (about 10.5g),

[0089] Cross-linking agent N, N'-methylenebisacrylamide (MBAA) 6.2g (about 59% of AA quality),

[0090] Porogen ethylene glycol 6.7mL (about 7.5g, about 71% of AA mass),

[0091] The consumption ratio of dispersant Span40 and Span80 is adjusted to 4.5 with the HLB v...

Embodiment 3

[0113] This embodiment is the immobilized porcine pancreatic lipase carrier prepared by the following method:

[0114] Surfactants Span (Span) 40 and Span80 are used as composite dispersants, kerosene is used as dispersed phase, acrylic acid (AA) is used as reactive monomer, and N,N'-methylenebisacrylamide (MBAA) is used as crosslinking agent , Ethylene glycol is the porogen, and the macroporous bead-shaped cross-linked polymer is prepared by reverse-phase suspension polymerization technology, and then activated by N-hydroxysuccinimide (NHS), to obtain the immobilized porcine pancreatic fat Enzyme carrier.

[0115] The raw materials used include:

[0116] Reactive monomer acrylic acid (AA) 2.5mL (about 2.6g),

[0117] Cross-linking agent N, N'-methylenebisacrylamide (MBAA) 1.3g (about 50% of AA quality),

[0118] Porogen ethylene glycol 2.3mL (about 2.6g, about 100% of AA mass),

[0119] The consumption ratio of dispersant Span40 and Span80 is adjusted to 6 with the HLB va...

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Abstract

The invention discloses an immobilized porcine pancreatic lipase carrier, which is a macroporous bead crosslinked polymer containing succinimidyl ester groups and has a structure represented by the formula I. The carrier forms a high polymer with acrylic acid as a reactive monomer and N,N'-methylenebisacrylamide as a crosslinking agent, and in the meantime, incorporates the succinimidyl ester active group so that the time required for immobilizing the porcine pancreatic lipase is short, namely only 1 hour is required, and the immobilizing efficient is high and stability of activity retention of the immobilized enzyme is excellent; the apparent activity of the immobilized enzyme is high and can reach 750U / g and above. The invention also discloses a preparation method and application of the immobilized porcine pancreatic lipase carrier.

Description

technical field [0001] The invention belongs to the technical field of preparation of immobilized enzyme carriers, in particular to an immobilized porcine pancreatic lipase carrier and a preparation method thereof. Background technique [0002] Enzymes are biocatalysts with remarkable characteristics such as strong specificity, high catalytic efficiency and mild action conditions. Therefore, it is widely used and can be used in many fields such as light industry, food, feed, medicine, chemical industry, environmental protection and energy, analysis and detection. However, due to the shortcomings of free enzymes that are difficult to separate from the product and are easily inactivated, people have tried various methods to modify enzyme molecules, one of which is the study of immobilized enzymes, thereby changing some of the properties and functions of enzymes. Make it overcome the shortcoming of free enzyme. The study of immobilized enzymes began in the 1950s. After more t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/06C08F220/56C08J9/14C12N11/08
Inventor 赵仕林粟慧君蔡莉
Owner SICHUAN NORMAL UNIVERSITY
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