Means and methods for diagnosing and/or treating a subject at risk of developing heart failure
A technology for heart failure and risk, applied in the fields of medicine and cardiology, can solve problems such as the inability to predict the outcome of heart failure and the inability to reliably prove heart failure
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example 1
[0057] Lysosomal integral membrane protein-2 is a new component of intercalary disc and prevents cardiomyopathy
[0058] Materials and methods
[0059] Ren-2 rat, microarray analysis and western blot
[0060] As mentioned earlier (Van Haaften et al., 2006), from ten-week-old Ren-2, Sprague-Dawley (SD) rats ( , Lille Skensveld, Denmark) took out a biopsy of LV. At 10 weeks, 12 weeks, 15 weeks, 16 weeks, 18 weeks, 19 weeks, and 21 weeks of age, the rats were subjected to continuous echocardiography. When the rats showed clinical signs of heart failure (heart failure tendency / HF Rats were sacrificed at 15 to 18 weeks when they were prone to rats); or when there were no clinical signs of exhaustion (compensated / comp rats), the rats were sacrificed at 21 weeks. As described earlier (Schroen et al., 2004; Heymans et al., 2005), total RNA was isolated from the LV biopsy and amplified, and the total RNA was hybridized to the Affymetrix rat 2302.0 gene chip and analyzed with Microarray ...
example 2
[0101] TGFβ promotes cardiac hypertrophy by inhibiting Krüppel-like factor 15, Krüppel-like factor 15 Is a new type of inhibitor of cardiac hypertrophy
[0102] Materials and methods
[0103] Transgenic rats, left ventricular biopsy and hemodynamic study
[0104] Eighteen male homozygous Ren-2 rats and five age-matched Sprague-Dawley (SD) ( Breeding Center, Lille Skensveld, Denmark). Three Ren-2 rats with clinical signs of heart failure were sacrificed at 10 to 12 weeks of age, and these three rats were excluded from the study. As mentioned earlier, at 10 weeks of age, biopsies of the left ventricle were taken from the remaining healthy 15 Ren-2 rats and 5 SD control rats. As described above, rats were subjected to continuous echocardiography at 10 weeks, 12 weeks, 15 weeks, 16 weeks, 18 weeks, 19 weeks, and 21 weeks of age. Nine Ren-2 rats with clinical signs of heart failure were sacrificed at 15 to 18 weeks of age and were designated as "prone to heart failure" rats. The r...
example 3
[0145] Krüppel-like factor 15, a transcription inhibitor of cardiac hypertrophy
[0146] According to the present invention, it has been shown that the zinc finger transcription factor Krüppel-like factor 15 (KLF-15) is an effective LV hypertrophy transcription inhibitor. Gene targeting studies have shown that KLF-15-deficient mice develop normally, but in response to pressure overload, they develop an expanded form of cardiac hypertrophy, which is characterized by increased heart weight, increased expression of hypertrophic genes, and increased muscle cell size. The left ventricular cavity dilates and the systolic function of the left ventricle decreases. In summary, these studies confirmed the role of KLF-15 in LV hypertrophy in vivo.
[0147] Interestingly, KLF-15 is down-regulated in several forms of pathological hypertrophy, but not in physiological hypertrophy, which means that KLF-15 is a regulator of pathological hypertrophy, not a regulator of physiological hypertrophy. ...
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