Fermentation method of microbial polysaccharide flocculant

A microbial polysaccharide and biological flocculant technology, applied in the field of modern microbial engineering, can solve the problems of long fermentation period, high raw material cost and production cost, waste of water resources, etc., and achieve the effect of strong flocculation function, large aggregation and adsorption capacity.

Inactive Publication Date: 2009-10-21
山东齐东方投资有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As far as the reported microbial flocculant-producing strains are concerned, there are deficiencies in varying de

Method used

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  • Fermentation method of microbial polysaccharide flocculant

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Embodiment 1

[0031] a) Preparation of fermentation medium

[0032] Mass composition of fermentation medium (g / L):

[0033] Starch sugar 20, (NH 4 ) 2 SO 4 4,K 2 HPO 4 6. MgSO 4 0.8, CaCl 2 1.3, corn syrup 1.3, water 1000.

[0034] Get 15% of the fermentation water for preparation (NH 4 ) 2 SO 4 Components, 85% of the fermentation water is used to prepare other components, which are respectively sterilized at 120°C for 8 minutes and then mixed in the fermenter for later use:

[0035] b) Fermentation method and fermentation condition control

[0036] A straight-leaf turbine-type ventilated fermenter is adopted, and the fermentation method adopts a cascaded strain proliferation fermentation method. The feeding coefficient of fermenter 1 is 75%, and the inoculation amount is 8%. Two stages of fermenters are connected in series, fermenter 1 is cultivated for 13 hours, 90% of the culture medium is transferred to fermenter 2, and fresh medium is added to fermenter 1 to re-prolifer...

Embodiment 2

[0040]a) The quality composition (g / L) of the preparation fermentation medium of fermentation medium:

[0041] Starch sugar 15, (NH 4 ) 2 SO 4 3,K 2 HPO 4 4. MgSO 4 0.5, CaCl 2 1.0, corn syrup 0.9, water 1000.

[0042] Get 15% of the fermentation water for preparation (NH 4 ) 2 SO 4 Components, 85% of the fermentation water is used to prepare other components, which are respectively sterilized at 120°C for 8 minutes and then mixed in the fermenter for later use:

[0043] b) Fermentation method and fermentation condition control

[0044] Straight-leaf turbine-type ventilated fermenter is adopted, and the fermentation method adopts the in-situ strain multiplication fermentation method, and the feeding coefficient of fermenter 1 is 70%, and the inoculation amount is 5%. Two stages of fermentors are connected in series, fermentor 1 is cultivated for 10 hours, 85% of the culture medium is transferred to fermentor 2, and fresh medium is added to fermentor 1 to re-prol...

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Abstract

The invention provides a fermentation method of a microbial polysaccharide flocculant, comprising the steps of choosing arthrobacter LF-Tou2 as a producing strain of the microbial polysaccharide flocculant and adopting cascaded strain propagation and fermentation methods to produce the high-efficient microbial polysaccharide flocculant used for water quality purification. The microbial polysaccharide flocculant of the invention is non-toxic and biodegradable, has the advantages of low production cost, wide application range, high service efficiency and no secondary pollution when being used, has comprehensive functions of removing inorganic ions, reducing contents of organic matters and adsorbing heavy metals, is applied in such technical fields as purification of quality of supplied water, treatment of inland lake water and sewage quality, separation of biological products in the fermentation industry and the like, and has very obvious economic and social effects.

Description

technical field [0001] The invention relates to a preparation method of a water treatment agent, which belongs to the technical field of modern microbial engineering, in particular to a fermentation method of a microbial polysaccharide flocculant. Background technique [0002] The fermentation methods used in the current microbial fermentation research and industrial production include batch culture, continuous culture and fed-batch culture methods, each with its own characteristics. Inorganic salt flocculants used in water supply and drainage, such as aluminum sulfate, aluminum chloride, ferric chloride, etc., have unsatisfactory treatment effects; polymeric inorganic salt flocculants, such as polyaluminum chloride, Polyferric sulfate, etc., have achieved good results in water treatment, but they will cause some secondary pollution to the environment during use, and the secondary pollution caused by aluminum is more obvious. High concentrations of aluminum ions in water ar...

Claims

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Application Information

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IPC IPC(8): C12P19/04C02F1/56C12R1/06
Inventor 栾兴社
Owner 山东齐东方投资有限公司
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