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Tissue engineering bone implant and method for constructing the same

A technology of bone graft and tissue engineering, applied in the construction and application of bionic artificial bone, can solve the problems of short half-life, difficult extraction, research and application limitations, etc., and achieve the effect of improving the success rate

Inactive Publication Date: 2009-10-28
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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Problems solved by technology

The safety and high-efficiency osteogenic activity of bone morphogenetic protein have been confirmed by more and more experiments. The results of a large number of studies on BMP and rhBMP by domestic and foreign scholars have shown that BMP-2 is considered to have the highest biological activity and is the most The promising osteoinductive protein, which can promote in situ and ectopic osteogenesis, is considered to be the most promising osteoinductive substance. The US Food and Drug Administration officially approved BMP-2 for the clinical treatment of long bone fractures in 2004, but due to The content of natural BMP-2 in the body is very small, the half-life is short, and the extraction is difficult, which limits the further research and application of BMP-2

Method used

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  • Tissue engineering bone implant and method for constructing the same
  • Tissue engineering bone implant and method for constructing the same
  • Tissue engineering bone implant and method for constructing the same

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Embodiment Construction

[0043] The present invention is described by examples below, and it should be noted that the examples listed should not be construed as limiting the invention.

[0044] As shown in the figure: a tissue engineered bone graft, including a scaffold material 1 and a seed cell 2 transfected with an adenovirus vector 3, the seed cell 2 is adhered to the scaffold material 1, forming a structure with a certain space Structure and bioactive cell carrier complex, the scaffold material 1 is nano-hydroxyapatite (Nano-HA) prepared by sol-flocculation method, on which bone marrow mesenchymal stem cells transfected with virus adhere.

[0045] Application of transgenic bionic artificial bone in animal bone defect repair:

[0046] 1. Animal experiment operation

[0047] 1. Choose Ketamine Hydrochloride Injection 20mg / kg, and inject it into anesthesia through the ear vein.

[0048] 2. The right forearm is routinely depilated, disinfected, and surgically draped.

[0049] 3. A 2 cm longitudina...

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Abstract

The invention provides a tissue engineering bone implant and a method for constructing the same, which includes stent material and seeded cell transfected with adenovirus vector. The seeded cell adheres to the stent material, and constitutes a cell vector complexes with a certain spatial structure and bioactivity. The stent material uses nano hydroxyapatite (Nano-HA) prepared by sol-flocculation method, and marrow mesenchymal stem cell with transfected virus is adhered to the nano hydroxyapatite. The tissue engineering bone provided by the invention, which includes marrow mesenchymal stem cell, adenovirus containing BMP-2 target gene and nano hydroxyapatite, and is constructed by a certain process and method, is bionic artificial bone with a certain spatial structure and bioactivity. The constructed bionic artificial tissue engineering bone provided by the invention can be used as bone implant for repairing bone with large defective segment, and has been proved to repair bone with large defective segment in animal experiment.

Description

technical field [0001] The invention relates to a repairing material for bone defects, which belongs to the technical field of constructing bionic artificial bone with gene-enhanced bone tissue engineering technology in biomedical tissue engineering, and specifically relates to the construction and application of a bionic artificial bone. Background technique [0002] Bone tissue defect caused by trauma, tumor, congenital deformity, infection, pathology and other factors is one of the clinical problems, and bone grafting is the main method to solve this problem. Bone grafting is mainly divided into autologous bone grafting, allogeneic or heterogeneous bone grafting. The drawbacks or limitations of these two methods mainly include insufficient supply sources, donor site damage, complications after bone harvesting, and graft rejection. In recent years, with the development of tissue engineering, tissue engineering bone grafts constructed using tissue engineering principles an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/12A61L27/56A61L27/54
Inventor 王大平何春耒熊建义刘黎军徐小平陆伟彭亮权刘建全李文翠朱伟民周可欧阳侃胡波
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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