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Vaccine product with respectively packaged freeze-dried vaccine and dilute solution thereof and method for preparing dilute solution

A solution and vaccine technology, applied in the field of preparation of the diluted compound solution, can solve the problems of shelf life limitation, application limitation, and no disclosed preparation method, etc., and achieve the effect of extending the shelf life and expanding the scope of application

Inactive Publication Date: 2009-12-02
LIAONING YISHENG BIOLOGY PHARMACY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Apparently, the polymyocytes containing kanamycin and calcium ions and the rabies virus antigen mentioned in this article are all liquid, and the vaccine made after the combination of the two is still a liquid vaccine, that is, an aqueous product, so that the shelf life of the product is limited. Strict restrictions, which in turn lead to the limited application of the product
Secondly, the paper does not disclose the preparation method of the adjuvant polymyocyte containing kanamycin and calcium ions

Method used

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  • Vaccine product with respectively packaged freeze-dried vaccine and dilute solution thereof and method for preparing dilute solution
  • Vaccine product with respectively packaged freeze-dried vaccine and dilute solution thereof and method for preparing dilute solution
  • Vaccine product with respectively packaged freeze-dried vaccine and dilute solution thereof and method for preparing dilute solution

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Embodiment Construction

[0029] Preparation of 0.5ml freeze-dried human rabies vaccine

[0030] Weigh out 1.0g of polyI and 1.0g of polyC respectively. Add about 800ml of 0.006mol / l phosphate buffer solution with a pH value of 8.0 to polyI and polyC respectively to dissolve, then combine the two liquids, put them in a water bath at 60±5°C, and react for 60 minutes while stirring at 100rpm. After the reaction was completed, 2 million units of kanamycin sulfate dissolved in 0.006 mol / l phosphate buffer solution with a pH value of 8.0 in 200 ml was added dropwise, and stirred at 100 rpm while adding dropwise. Finally, 8ml of 0.1mol / L calcium chloride solution was added dropwise. After the sample is stirred evenly, add 0.006mol / l phosphate buffer solution to a final volume of 2000ml, stir again, filter through a 0.45μm microporous filter membrane in a sterile environment, and dispense. The aliquoted samples were sterilized by circulating steam at 105°C for 30 minutes.

[0031] In a sterile environment,...

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Abstract

The invention provides a vaccine product with respectively packaged freeze-dried vaccine and dilute solution thereof and method for preparing the dilute solution, which aims to solve the problem that the patent No. 93105862.7 records a vaccine in liquid state made by the combination of polyinosinic cytidilic acid containing kanamycin and calcium ions with lyssa virus antigen, both of which are in liquid states, has a guarantee period that is strictly limited to result in the limitation of the application of the product; and the method for preparing the adjuvant of polyinosinic cytidilic acid containing kanamycin and calcium ions is not disclosed in the patent No. 93105862.7. The invention has the following essentials: (1) freeze-dried vaccine and dilute solution thereof are respectively packaged, and the dilute solution is the polyinosinic cytidilic acid containing kanamycin and calcium ions; (2) the method for preparing the dilute solution. The invention overcomes the defect that the freeze-dried vaccine can not use PIKA adjuvant, and widens the practical application range of the PIKA adjuvant.

Description

technical field [0001] The invention relates to a rabies vaccine product for human use. Specifically, the vaccine product is a freeze-dried vaccine and its diluted compound solution packaged separately and bundled for sale. The invention also relates to a preparation method of the diluted compound solution. Background technique [0002] The Chinese Patent Gazette discloses a patent specification (hereinafter referred to as the prior art) that the patent number is 93105862.7, and the name is "polymyocyte complex immune adjuvant and a vaccine containing the adjuvant". This article mainly records that polymyocytes containing kanamycin and calcium ions are combined with rabies virus antigen to make a vaccine, and immunizing OF1 mice can significantly improve the immune effect of the antigen or vaccine. Apparently, the polymyocytes containing kanamycin and calcium ions and the rabies virus antigen mentioned in this article are all liquid, and the vaccine made after the combinatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K9/19A61P31/14A61K39/205
Inventor 张译胡金东苏文全李艳
Owner LIAONING YISHENG BIOLOGY PHARMACY
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