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Application of small RNAs in preparing medicaments for treating and/or preventing lymphoma

A lymphoma, tiny technology, applied in the field of application in the preparation of drugs for the treatment and/or prevention of lymphoma, to achieve good curative effect, inhibit the growth of tumor cells, and promote the effect of tumor cell apoptosis

Inactive Publication Date: 2009-12-02
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Existing studies have shown that miR-15a / 16-1 may be related to the occurrence of hematological tumors, but there is no evidence that these two microRNAs can be used for tumors, especially lymphoma treatment and / or prevention

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  • Application of small RNAs in preparing medicaments for treating and/or preventing lymphoma
  • Application of small RNAs in preparing medicaments for treating and/or preventing lymphoma
  • Application of small RNAs in preparing medicaments for treating and/or preventing lymphoma

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Embodiment Construction

[0053] The present invention will be described in further detail below in conjunction with the embodiments, but the implementation of the present invention is not limited thereto.

[0054] 1. Experimental method

[0055] 1. Design and synthesis of miR-15a, miR-16-1 and pre-miR-15a (miR-15a precursor) oligonucleotides

[0056] According to MiRBase( http: / / www.sanger.ac.uk / Software / Rfam / mirna) to obtain the sequences of human miR-15a, miR-16-1 and pre-miR-15a. The BLAST software was used to analyze and determine the random control sequence. The miR-15a and miR-16-1 oligonucleotides were single-stranded full-thio-modified synthesized by Shanghai Bioengineering Technology Service Co., Ltd. The sequence looks like this:

[0057] Three RNAs chemically synthesized:

[0058] miR-15a: 5'-uagcagcacauaauguuugug-3' (22bp)

[0059] miR-16-1: 5'-uagcagcacguaaauauuggcg-3' (22bp)

[0060] scrambled sequence: 5’-cauuaaugucggacaacucaau-3’ (22bp)

[0061] pre-miR-15a (corresponding gene ...

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Abstract

The invention relates to application of small RNAs in the field of preparing medicaments for treating and / or preventing lymphoma, in particular to the application of small RNA-15a, small RNA-16-1, precursor of the small RNA-15a and precursor of the small RNA-16-1 or a mixture of more than two of the small RNA-15a, the small RNA-16-1, the precursor of the small RNA-15a and the precursor of the small RNA-16-1 in preparing the medicaments. The over expression of the oligonucleotide array sequences in lymphomatous cells proves that the oligonucleotide array sequences have effects of inhibiting the growth of the lymphomatous cells and promoting the apoptosis of the lymphomatous cells and further discloses that the use of the oligonucleotide array sequences in combination with chemotherapeutics such as cytarabine can strengthen the efficacy of original medicaments, thereby ensuring the application potential of the oligonucleotide array sequences in the field of preparing the medicaments for treating and / or preventing lymphoma. The medicaments can be a single composite containing an oligonucleotide array sequence or mixture of the oligonucleotide array sequences and a pharmaceutically carrier or a chemotherapeutic-containing composite for use in combination.

Description

technical field [0001] The invention relates to the field of microRNA and its application, in particular to the application of microRNA-15a (miR-15a) and microRNA-16-1 (miR-16-1) in the preparation of drugs for treating and / or preventing lymphoma. Background technique [0002] miRNA is a regulatory molecule in the process of gene expression and protein translation, and plays a pivotal role in the regulation of tumorigenesis. Some miRNAs may be potential oncogenes or tumor suppressor genes. By searching for miRNAs with properties of oncogenes and tumor suppressor genes, new targets can be provided not only for the diagnosis of tumors but also for the biological treatment of tumors. [0003] miRNA-related tumor gene therapy includes knocking out or knocking down oncogene miRNA and introducing tumor suppressor gene miRNA. On the one hand, the introduction of synthetic antisense oligonucleotides (Anti-miRNAAntisense Oligonucleotides, AMOs) complementary to miRNAs with oncogene ...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K31/7068A61K31/475A61K31/704A61K31/519A61K33/36A61P35/00
Inventor 何冬梅谌琴
Owner JINAN UNIVERSITY
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