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Method for embryo culture in vitro and plant regeneration of euscaphis konishii hayata

The invention relates to a technology for cultivating the embryos of C. chinensis, which is applied in the directions of plant regeneration, botanical equipment and methods, horticultural methods, etc., and can solve the problems of short proliferation time, low seed germination rate, inability to provide tissue culture seedlings in large quantities, and the like, To achieve the effect of good growth and significant economic benefits

Inactive Publication Date: 2011-07-20
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a tissue culture method for in vitro culture and plant regeneration of C. sylvatica embryos, to solve the problem of the low germination rate of C. sylvanis in the prior art and the inability to provide a large number of excellent purified components The problem of cultivating seedlings, the buds cultivated by this method are healthy, strong, and the proliferation time is short, and the survival rate of mature seedlings after transplantation is high

Method used

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  • Method for embryo culture in vitro and plant regeneration of euscaphis konishii hayata
  • Method for embryo culture in vitro and plant regeneration of euscaphis konishii hayata
  • Method for embryo culture in vitro and plant regeneration of euscaphis konishii hayata

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Embodiment

[0021] Material collection method: select the seeds with full grains of the year, and wrap them with a damp cloth after picking;

[0022] Medium:

[0023] The MS basic medium was prepared using the formula in "Tissue Culture and Application of Woody Plants" published by Beijing Higher Education Press and edited by Chen Zhenghua in 1986.

[0024] The preparation of culture medium: basic medium is MS, add growth hormone to be BA, NAA, IAA, IBA, also add Su (sucrose) to be 20g / L in the medium, Ag + 7.5g / L, PH value is 5.8, the additive is activated carbon Ac; the thickness of the medium is generally 1.4 ~ 1.6cm;

[0025] Bud induction medium→MS+BA(1.0mg / L)+NAA(0.5 mg / L)+IAA(1.0mg / L)+Ag + (7.5g / L)+Su(20g / L)+Ac(2~2.5g / L)

[0026] Bud Proliferation Medium→MS+BA(2.0mg / L)+NAA(0.3mg / L)+IAA(1.0mg / L)+Ag + (7.5g / L)+Su(20g / L)+Ac(2~2.5g / L)

[0027] Rooting medium→1 / 2MS+NAA(0.1mg / L)+IBA(0.5mg / L)+Ag + (7.5g / L)+Su(20g / L)+Ac(2~2.5g / L)

[0028] Ag in medium + Derived from AgNO 3 .

[0...

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Abstract

The present invention provides a method for embryo culture in vitro and plant regeneration of euscaphis konishii hayata, and belongs to the technique of culturing euscaphis konishii hayata embryo. The method solves the problems existing in the prior art that the seed germination rate of the euscaphis konishii hayata embryo is low, and well purified tissue culture seedlings cannot be provided in large amount. In the method, the full seeds in the same year are selected as materials, and the embryo undergoes induced culture for 15 to 20 days to grow strong seedlings; after proliferation for 20 to 30 days and induced rooting for 20 to 25 days, the seedlings are grown and transplanted, and the survival rate is as high as more than 95 percent; and the propagation factor is higher, and the survived seedlings are tall and strong, and well grown.

Description

technical field [0001] The invention belongs to the cultivation technique of the embryos of Crows rotundum, and more specifically relates to a method for cultivating and regenerating the isolated embryos of Crows sliver in vitro. Background technique [0002] Eascaphis kohlshli Hayata (Eascaphis kohlshli Hayata) belongs to the genus Eascaphis kohlshli Hayata, also known as Eascaphis kohlshli Hayata, Eascaphis kohlshli Hayata, and Eascaphis kohlshli Hayata. It is a unique native ornamental tree species in my country. It blooms in summer, bears fruit in winter, and has a long fruit period. When the red fruit matures, it cracks along the ventral suture. Together with its black seeds, it looks like a beautiful and lifelike red butterfly. It has great ornamental value and can be used as a landscape tree or street tree. The fruit-hanging time is very long, from the exocarp turning red to the peel off, the time is as long as half a year. Therefore, it can be viewed for a long time...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/08A01G31/00A01H4/00
Inventor 何碧珠邹双全李玉平
Owner FUJIAN AGRI & FORESTRY UNIV