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Test strip for fast testing gossypol and preparation method and application thereof

A technology for detecting test paper and gossypol, applied in the field of gossypol detection test paper and preparation, can solve the problems of unfavorable popularization, expensive instruments, strong professionalism and the like, and achieves the effect of being beneficial to popularization and popularization.

Inactive Publication Date: 2009-12-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above detection methods for gossypol have their own advantages and disadvantages. Some detection methods have high sensitivity, and some detection methods have good selectivity, but there are disadvantages such as expensive instruments, complicated operation, strong professionalism, and unfavorable popularization.

Method used

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  • Test strip for fast testing gossypol and preparation method and application thereof
  • Test strip for fast testing gossypol and preparation method and application thereof
  • Test strip for fast testing gossypol and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of embodiment 1 gossypol kit and standard color band

[0044] Reagents required for the preparation of gossypol (Gossypol) kit: iron acetylacetonate (Fe(acac) 3 ) and o-phenoline, as well as solvent ethanol, glacial acetic acid and hydrochloric acid, etc. Weigh 0.353g of iron acetylacetonate and dissolve it in 100ml ethanol. The molar concentration of iron acetylacetonate is 0.01mol / L, and 10ml of it is diluted to 0.002mol / L; 0.36g of phenoline is dissolved in 100ml of hydrochloric acid and ice In the mixed acid solution of acetic acid, the mass percentage of the solution is 0.36%. Take 0.02mol / L iron acetylacetonate 1ml, 0.36% o-phenoline 1ml and ethanol 1ml respectively and put them into a 5ml colorimetric tube and mix evenly to obtain the gossypol kit.

[0045] (1) Effect of the volume ratio of iron acetylacetonate and gossypol on color reaction

[0046] The volume ratio of iron acetylacetonate and gossypol is 1:1-4 respectively, and the UV-vis abs...

Embodiment 2

[0052] Embodiment 2: Utilize the kit of the present invention to detect the content of gossypol in cottonseed oil

[0053] Weigh 2g of cottonseed oil into an Erlenmeyer flask, add 5ml of methanol at the same time, cover the bottle, keep away from light at room temperature, oscillate and extract for 30min to obtain the sample to be tested. Get 1ml of the sample to be tested and put it into the gossypol kit prepared according to the method described in Example 1, and develop a color reaction for 2min (such as Figure 6 a). By comparing with the standard color band of the kit, the colorimetric value is between 0-0.01%, and substituting the gossypol mass percentage content or range X%=(AρV) / M formula to obtain the content range of gossypol in cottonseed oil 0-0.02%. This sample adopts UV-vis spectrophotometry to detect gossypol, records its absorbance value as 0.33A, from gossypol standard curve ( Figure 4 ), its concentration was found to be 61ug / ml, and the content of gossyp...

Embodiment 3

[0054] Embodiment 3: Utilize the kit of the present invention to detect the content of gossypol in cottonseed kernel

[0055] The extraction of gossypol in cottonseed kernel was carried out with reference to the method of Example 2. Weigh 2g of cottonseed kernels, pulverize them and put them into an Erlenmeyer flask, add 5ml of methanol at the same time, cover the bottle, and keep away from light at room temperature, shake and extract for 40min to obtain gossypol extract. Get gossypol extract 1ml and put it into the gossypol kit prepared according to the method described in Example 1, and develop a color reaction for 2min (such as Figure 6 b). By comparing with the standard color band of the kit, the colorimetric value is between 0.15%-0.20%, and substituting the gossypol mass percentage content or range X%=(AρV) / M formula to obtain the gossypol content in cottonseed oil The range is 0.30%-0.40%. Get above-mentioned gossypol extract 1ml to dilute 10 times and adopt UV-vis ...

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Abstract

The invention belongs to the technical field of chemical test and analysis, and in particular relates to a test strip suitable for fast testing a gossypol, and a preparation method and an application thereof. The preparation process of the test strip of the invention includes the preparation of a test reagent box and a standard gossypol colorimetric card which is matched with the box to use. The core reagent includes color development reagent, namely acetylacetone iron or ferric trichloride or iron sulfate, a mixed acid solution of hydrochloric acid and glacial acetic acid, complexing agent, namely o-phenanthroline and immobilization agent ludox or soluble starch. The standard gossypol colorimetric card which is matched with the test strip of the invention to use displays the color by a 10-point standard gossypol solution and the test strip, and has different color grades. The test strip of the invention is applicable to the fast test of the gossypol in various cottonseed products, and has the outstanding advantages of simple method, sensitive test and convenient popularization.

Description

technical field [0001] The invention relates to the technical field of chemical testing and analysis, in particular to a gossypol detection test strip and its preparation method and application. technical background [0002] Gossypol, also known as gossypol or gossypol, is a yellow polyphenolic substance in cotton plants, which exists in various organs of cotton plants, with the highest content in cottonseed kernels. The molecular formula of gossypol is C 30 h 30 o 8 , its molecular structure contains very active hydroxyl and aldehyde groups. Gossypol is soluble in organic solvents such as methanol, ethanol, and acetone, but insoluble in water and low-boiling petroleum ether. Gossypol is weakly acidic and can react with aqueous solutions of sodium hydroxide and potassium carbonate to form disodium or dipotassium salts. At the same time, it is easily oxidized by many oxidants such as hydrogen peroxide and Fehling's reagent, and even deteriorated by oxidation by oxygen in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/22G01N21/33
Inventor 韩鹤友胡圣扬王运
Owner HUAZHONG AGRI UNIV
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