Technical method of triploid seed production of rainbow trout

A technology for rainbow trout and triploids, which is applied in fish farming, agricultural fishing, climate change adaptation, etc., and can solve the problems that there is no productive test and put into production.

Active Publication Date: 2009-12-23
北京顺通虹鳟鱼养殖中心
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  • Summary
  • Abstract
  • Description
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  • Application Information

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  • Technical method of triploid seed production of rainbow trout
  • Technical method of triploid seed production of rainbow trout
  • Technical method of triploid seed production of rainbow trout

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Embodiment 1

[0055] Embodiment 1: as figure 1 Shown, the technical solution that the present invention solves its technical problem adopts is:

[0056] At an appropriate time after the egg and sperm are combined, heat (shock) the fertilized eggs in a low temperature environment within a certain time interval, and then return to the original low temperature environment for cold water incubation. More than 80% of the fertilized eggs that should be bred into diploids will produce triploid fertilized eggs after heat shock treatment. Triploid seedlings (juveniles) are then bred from triploid fertilized eggs.

[0057] △Three key technical parameters of technical means

[0058] Parameter (1), start timing of heat (shock) treatment: at 10°C water temperature, heat (shock) treatment was started on fertilized eggs 10 minutes after artificial insemination.

[0059] Parameter (2), heat shock temperature: 28-30°C.

[0060] Parameter (3), heat shock time threshold: 10-12 minutes.

[0061] △ Key equi...

Embodiment 2

[0081] Rainbow trout triploid breeding process:

[0082] Step 1, artificially collect eggs, collect sperm, wash eggs with mono-salt isotonic solution, and artificially inseminate.

[0083] Step 2, put the fertilized eggs into a heat-induced incubator, implement cold running water (10° C.) to absorb water and expand, and at the same time, excess semen overflows with the water flow.

[0084] Step 3. After 10 minutes, stop injecting cold water and replace it with hot water at 28-30°C to induce triploid fertilized eggs.

[0085] Step 4. After 10 minutes, stop injecting hot water, open the bottom drain, and then inject water at 10°C to implement cold water absorption and expansion and hatching operations.

[0086] Steps: before and after the hatching accumulated temperature reaches 220°C·d, move the eyed eggs into the juvenile hatching tank until the juveniles hatch.

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Abstract

The invention relates to a technical method of triploid seed production of rainbow trout. The technical method comprises the following steps: in proper time after sperm-egg binding, carrying out thermal (shock) treatment on oosperm in low-temperature environment in a certain time interval, then restoring the original low-temperature environment and carrying out cooling water incubation; leading more than 80% of oosperms which need to be incubated into diploid oosperm to generate triploid oosperm after thermal shock treatment; and raising the triploid oosperm into triploid offspring seed (larval fish). The implementation of the technical proposal stops the discharging of a secondary polar body of the oosperm, induces the oosperm to produce another type of oosperm which is fused by the egg, the sperm nucleus and the secondary polar body with respective monoploid chromosome and contains triploid chromosome, namely triploid oosperm, and realize industrialization of triploid seed production. Each thermal inducing incubator can produce more than 40,000 of triploid oosperms for one time, and each reproduction period (October to February) can produce more than 2,000,000 oosperms. The inductivity of the triploid is more than 80%.

Description

technical field [0001] The invention relates to a rainbow trout triploid breeding technique and belongs to the technical field of fish chromosome engineering breeding. Background technique [0002] Since German biologists discovered the phenomenon that rays can control the number of chromosomes in fertilized cells of frogs, ichthyologists in Europe, the United States and Japan have further developed artificial methods to control the behavior, quantity, and nature of chromosomes in fertilized cells in fish, and then breed new species. , that is, chromosome engineering breeding technology. This is a high-tech that is different from traditional genetic breeding. [0003] The key point of the invention is to adopt the heat shock method, that is, the heat treatment method, to prevent the discharge of the second polar body of fertilized eggs of rainbow trout, a cold-water fish, and induce the production of triploid fertilized eggs. At present, there is no comparable technical ex...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81Y02P60/60
Inventor 沈希顺王昭明杨玛丽
Owner 北京顺通虹鳟鱼养殖中心
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