Sample injection device of high performance liquid chromatography-tandem mass spectrometer

Abstract

The invention relates to a high performance liquid chromatography-tandem mass spectrometer, in particular to a sample injection device of a high performance liquid chromatography-tandem mass spectrometer. the device comprises a mobile phase container, a needle washing liquid container, an injector, a sample injection tube, a sample injection needle, a needle stand and a six-way valve; the sample injection tube is provided with a three-way valve connected with the injector; the sample injection needle is connected with the three-way valve through the sample injection tube; the three-way valve is connected with the mobile phase container through a mobile phase tube; a degasser is connected with the mobile phase container; the three-way valve is additionally connected with the needle washingliquid container through a needle washing tube. The sample injection device has following advantages: firstly, vacuum degassing is not required for the mobile phase tube by a vacuum degasser with complex structure, the arrangement of the vacuum degasser is relieved, pipelines are distributed in order, and convenience is brought to the maintenance and the replacement of the pipelines; secondly, background interference can be effectively reduced during the detection and analysis of fluorinated alkyl compounds.

Description

technical field [0001] The invention relates to a high-performance liquid chromatography-tandem mass spectrometer, in particular to a sampling device of the high-performance liquid chromatography-tandem mass spectrometer. Background technique [0002] At present, high performance liquid chromatography-tandem mass spectrometry is widely used in compound detection and analysis because of its high selectivity and high sensitivity. Existing high performance liquid chromatography-tandem mass spectrometer, its vacuum degasser is made of a four-channel vacuum box and a vacuum pump, after turning on the power switch of the vacuum degasser, the control circuit turns on the vacuum pump, and the operation of the vacuum pump causes the vacuum box to generate Partial vacuum, when the solvent passes through the channel in the vacuum box, the dissolved gas in the solvent will permeate through the plastic film and enter the vacuum box to achieve the purpose of degassing. Such operations br...

AI Technical Summary

Problems solved by technology

Existing high performance liquid chromatography-tandem mass spectrometer, its vacuum degasser is made of a four-channel vacuum box and a vacuum pump, after turning on the power switch of the vacuum degasser, the control circuit turns on the vacuum pump, and the operation of the vacuum pump causes the vacuum box to generate Partial vacuum, when the solvent passes through the channel in the vacuum box, the dissolved gas in the solvent will permeate through the plastic film and enter the vacuum box to achieve the purpose of degassing. Operations such as this will bring trouble and easily cause the modified degasser to fail to work normally.

[0003] In addition, the existing high-performance liquid chromatography-tandem mass spectrometer also has the following disadvantages: it will produce interference when detecting and analyzing perfluoroalkyl compounds, which will affect the detection results

Ordinary high performance liquid chromatography-tandem mass spectrometer is composed of high performance liquid chromatography and mass spectrometry. In the high performance liquid chromatography part, due to the existence of Teflon (polytetrafluoroethylene) components, the actual sample In the analysis, there will be background interference in the analysis of perfluorinated compounds, especially for perfluorocarboxylic acids, such as PFDoDA, PFUnDA, PFDA, PFNA, PFOA, PFHpA, PFHxA, etc., the background value is at the level of 0.5-1.5ng / mL, Seriously affect the accurate quantification of detection analysis

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