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Method and device for the quantitative real time analysis of fluorescent samples

A real-time analysis and sample technology, applied in the direction of measuring devices, analysis materials, fluorescence/phosphorescence, etc., can solve the problem of time-consuming analysis and achieve the effect of reducing the time period

Inactive Publication Date: 2012-06-27
EPPENDORF AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To obtain a light intensity high enough to e.g. determine the amount of DNA (e.g. in PCR) requires many repeated cycles in which the DNA is replicated, making the analysis often very time consuming

Method used

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  • Method and device for the quantitative real time analysis of fluorescent samples
  • Method and device for the quantitative real time analysis of fluorescent samples
  • Method and device for the quantitative real time analysis of fluorescent samples

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Embodiment Construction

[0021] exist figure 1 A block diagram of an apparatus that can be used for quantitative real-time analysis of fluorescent samples is shown in . As an example, the device is used with polymerase chain reaction (PCR) to determine the amount of DNA formed. Thus, multiple samples with DNA molecules to be replicated or amplified, respectively, are heated and annealed in repeated three-stage cycles as described above so that the fluorescent dye binds to the double-stranded DNA and fluoresces when excited by external light . To determine the amount of DNA replicated in each cycle in the sample, the intensity of light emitted by the sample is measured by the device.

[0022] The device provides a tray with a plurality of small containers 11 filled with samples with DNA molecules, primers, nucleotides and fluorescent dyes. The trays are alternately heated and annealed within the cycle described above. An electrical light source 12 is assigned to each container 11 in the tray, capab...

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Abstract

A method for the quantitative real time analysis of fluorescent samples is provided, at which the samples are excited to fluoresce by a sample individual light source (12) and the intensity of the light which is emitted by the samples is measured. For a highly precise measurement of even low light intensities for the purpose of reduction of the analysis time, each light source (12) is switched onand off during a defined interval by a clocked pulse sequence of constant pulse frequency alternately. The measurement of the intensity of the emission light during these intervals is exclusively performed during the switch-on phases of the light source (12).

Description

technical field [0001] The invention relates to a method and a device for the quantitative real-time analysis of fluorescent samples according to the preamble of claim 1 . Background technique [0002] In combination with the polymerase chain reaction, the so-called PCR (Polymerase Chain Reaction), this method is used especially in clinical diagnostics to determine the amount of DNA (deoxyribonucleic acid). According to the repeated cycle, in order to be copied or amplified respectively, using the primer (primer) as the starting DNA and the nucleotides bound to the primer, the DNA molecule sample is heated to 95°C in the first step of the cycle, so that The complementary strand of DNA is denatured. By lowering the temperature to 55°C in the second step of the cycle, hybridization occurs in which the primers bind to the DNA. In the third step of the cycle, the sample is heated to 72°C. At this working temperature, the polymerase further assembles nucleotides onto the growi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6452Y10T436/143333
Inventor N·维特席夫R·特雷普托G·J·埃克特A·席尔
Owner EPPENDORF AG
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